Library Subscription: Guest
Begell Digital Portal Begell Digital Library eBooks Journals References & Proceedings Research Collections
International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v18.i1.80
pages 67-74

A Lectin Purified from Blood Red Bracket Mushroom, Pycnoporus sanguineus (Agaricomycetidae), Mycelium Displayed Affinity Toward Bovine Transferrin

Silvana Alborés
Laboratorio de Microbiologia, Departamento de Biociencias, Facultad de Quimica, Universidad de la Republica, Montevideo, Uruguay
Maria Moros
Instituto de Nanociencia de Aragon, Universidad de Zaragoza, Zaragoza, Espana
María Pía Cerdeiras
Laboratorio de Microbiologia, Departamento de Biociencias, Facultad de Quimica, Universidad de la Republica, Montevideo, Uruguay
Jesus Martinez de la Fuente
Instituto de Ciencia de Materiales de Aragon, CSIC, Zaragoza, Espana
Valeria Grazu
Instituto de Nanociencia de Aragon, Universidad de Zaragoza, Zaragoza, Espana
Laura Franco Fraguas
Catedra de Bioquimica, Departamento de Biociencias, Facultad de Quimica, Universidad de la Republica, Montevideo, Uruguay

ABSTRACT

Fungal lectins constitute excellent ligands for development of affinity adsorbents useful in affinity chromatography. In this work, a lectin was purified from Pycnoporus sanguineus (PSL) mycelium using 3 procedures: by affinity chromatography, using magnetic galactosyl-nanoparticles or galactose coupled to Sepharose, and by ionic exchange chromatography (IEC). The highest lectin yield was achieved by IEC (55%); SDS-PAGE of PSL showed 2 bands with molecular mass of 68.7 and 55.2 kDa and IEC displayed 2 bands at pi 5.5 and 5.2. The lectin agglutinates rat erythrocytes, exhibiting broad specificity toward several monosaccharides, including galactose. The agglutination was also inhibited by the glycoproteins fetal calf fetuin, bovine lactoferrin, bovine transferrin, and horseradish peroxidase. The lectin was then used to synthesize an affinity adsorbent (PSL-Sepharose) and the interaction with glycoproteins was evaluated by analyzing their chromatographic behaviors. The strongest interaction with the PSL-derivative was observed with transferrin, although lower interactions were also displayed toward fetuin and lactoferrin. These results indicate that the purified PSL constitutes an interesting ligand for the design of affinity adsorbents to be used (i.e., in glycoprotein purification).


Articles with similar content:

Chemical Composition and Medicinal Value of the New Ganoderma tsugae var. jannieae CBS-120304 Medicinal Higher Basidiomycete Mushroom
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 8
Solomon P. Wasser, Lital E. Sharvit, Mikheil D. Asatiani, Gayane S. Barseghyan, Jannie Siew Lee Chan, Beny Trabelcy
Purification and Characterization of a Novel Protease from the Inky Cap Mushroom, Coprinopsis atramentaria (Agaricomycetes)
International Journal of Medicinal Mushrooms, Vol.20, 2018, issue 4
Hexiang Wang, Wenwen Chen, Xueran Geng, Tzi Bun Ng, Weiwei Zhang
Isolation and Characterization of a Ubiquitin-Like Ribonuclease from the Cultured Deep Root Mushroom, Oudemansiella radicata (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 11
Hao Chen, Hexiang Wang, Qin Liu, Tzi Bun Ng
The Impact of Drying of Wild-Growing Mushrooms on the Content and Antioxidant Capacity of Water- Soluble Polysaccharides
International Journal of Medicinal Mushrooms, Vol.21, 2019, issue 4
Katarzyna Skrzypczak, Monika Michalak-Majewska, Wojciech Radzki, Aneta Slawinska
A Study of Pharmacological Components from Submerged Culture Mycelia of Phellinus linteus (Berk. et Curt.) Teng
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 3
Yukihito Akiyama, Tomoyuki Nakamura