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International Journal of Medicinal Mushrooms
CiteScore™: 1.38 IF: 1.211 5-Year IF: 1.394 SNIP: 0.661 SJR: 0.433

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.2017021225
pages 685-696

Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), Inhibits Candida Biofilms: A Metabolomic Approach

Anuja Bhardwaj
Defence Institute of Physiology and Allied Sciences, DRDO, Timarpur, Delhi, India
Payal Gupta
Department of Biotechnology, Graphic Era University, Dehradun, India
Navin Kumar
Department of Biotechnology, Graphic Era University, Dehradun, India
Jigni Mishra
Defence Institute of Physiology and Allied Sciences, DRDO, Timarpur, Delhi, India
Ajai Kumar
Advanced Instrumentation Research Facility, Jawaharlal Nehru University, New Delhi, India
Rakhee
Defence Institute of Physiology and Allied Sciences, DRDO, Timarpur, Delhi, India
Kshipra Misra
Department of Biochemical Sciences (DBCS) Defense Institute of Physiology and Allied Sciences( DIPAS) Lucknow Road, Timarpur Delhi-110054

ABSTRACT

This article presents a comparative gas chromatography (GC)−mass spectrometry (MS)−based metabolomic analysis of mycelia and fruiting bodies of the medicinal mushroom Ganoderma lucidum. Three aqueous extracts−mycelia, fruiting bodies, and a mixture of them−and their sequential fractions (methanolic and ethyl acetate), prepared using an accelerated solvent extractor, were characterized by GC-MS to determine volatile organic compounds and by high-performance thin-layer chromatography to quantify ascorbic acid, a potent antioxidant. In addition, these extracts and fractions were assessed against Candida albicans and C. glabrata biofilms via the XTT reduction assay, and their antioxidant potential was evaluated. Application of chemometrics (hierarchical cluster analysis and principal component analysis) to GC data revealed variability in volatile organic compound profiles among G. lucidum extracts and fractions. The mycelial aqueous extract demonstrated higher anti-Candida activity and ascorbic acid content among all the extracts and fractions. Thus, this study illustrates the preventive effect of G. lucidum against C. albicans and C. glabrata biofilms along with its nutritional value.