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International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v15.i1.90
pages 81-90

Production of Biomass and Polysaccharides of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (W.Curt. :Fr.) P. Karst. (Higher Basidiomycetes), by Submerged Cultivation

Jozica Habijanic
National Institute of Chemistry, Ljubljana, Slovenia ; Faculty of Chemistry and Chemical Technology, University of Ljubljana, Ljubljana, Slovenia
Marin Berovic
Faculty of Chemistry and Chemical Engineering Department of Chemical and Biochemical Engineering University of Ljubljana Askerceva 5, 1001 Ljubljana, Slovenia
Bojana Boh
Faculty of Natural Sciences and Engineering, University of Ljubljana, Ljubljana, Slovenia
Branka Wraber
Institute of Microbiology and Immunology, Medical Faculty, University of Ljubljana, Ljubljana, Slovenia
Vlatka Petravic-Tominac
Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia

ABSTRACT

Submerged batch and repeated fed-batch cultivation techniques were used for mycelia cultivation and polysaccharide production of the Lingzhi or Reishi medicinal mushroom Ganoderma lucidum. Although most publications use various Asiatic G. lucidum strains, the growth of the strain Ga.l 4 (Biotechnical Faculty Strain Collection, Ljubljana, Slovenia), originally isolated from the Slovenian forest, is much faster. The results between the batch and repeated fed-batch cultivation are compared with the polysaccharide production in batch cultivation. From the aspect of biomass production, the best results were obtained in repeated fed-batch after 44 days, where 12.4 g/L of dry fungal biomass was obtained.


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