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International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v15.i1.70
pages 57-69

Chemical Characterization and Structure of Exopolysaccharides from Submerged Culture of New Medicinal Mushroom from China, Phellinus mori (Higher Basidiomycetes)

Chunlei Cao
Institute of Microbiology, Beijing Forestry University
Feng Peng
Institute of Biomass Chemistry and Technology, Beijing Forestry University, Beijing, China
Bao-Kai Cui
Institute of Microbiology, Beijing Forestry University, Beijing, China

ABSTRACT

Two different exopolysaccharides (EPSs) were first time-fractionated from submerged mycelia culture of Phellinus mori (PM) by gel-filtration chromatography: PM-EPS1 and PM-EPS3. Results of compositional analysis using high-performance anion exchange chromatography indicated that both PM-EPS1 and PM-EPS3 were heteropolysaccharides consisting of mannose, glucose, galactose, and rhamnose. Gel permeation chromatography analysis showed that the weight-average molecular mass of PM-EPS1 and PM-EPS3 were 4,990 g/mol and 27,890 g/mol, respectively. Fourier transform infrared spectral analysis of PM-EPS1 and PM-ESP3 revealed prominent characteristic groups corresponding to carbohydrates. 1H and 13C nuclear magnetic resonance spectroscopy analysis indicated that PM-EPS3 had a (1→4)-linked mannopyranosyl backbone, with branches of (1→4)-linked glucosyl residues and (1→3, 4)-linked galactopyranosyl residues. Preliminary activity tests in vitro revealed that the hydroxyl radical scavenging activity and total superoxide dismutase activities of PM-EPS3 obviously increased with an increase in concentration at the range 0.1−2.5 mg/mL.