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International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v12.i3.30
pages 245-255

Infuence of Culinary-Medicinal Maitake Mushroom, Grifola frondosa (Dicks.: Fr.) S.F. Gray (Aphyllophoromycetideae) Polysaccharides on Gene Expression in Jurkat T Lymphocytes

Natasa Radic
Department of Pharmaceutical Biology, Faculty of Pharmacy, University of Ljubljana, Askerceva 7, Ljubljana, Slovenia
Zala Jevnikar
Department of Pharmaceutical Biology, Faculty of Pharmacy, University of Ljubljana, Ljubljana, Slovenia
Natasa Obermajer
Department of Pharmaceutical Biology, Faculty of Pharmacy, University of Ljubljana; and Department of Biotechnology, Jozef Stefan Institute, Ljubljana, Slovenia
Jernej Kristl
Department of Pharmaceutical Biology, Faculty of Pharmacy, University of Ljubljana, Ljubljana, Slovenia
Janko Kos
Department of Pharmaceutical Biology, Faculty of Pharmacy, University of Ljubljana; and Department of Biotechnology, Jozef Stefan Institute, Ljubljana, Slovenia
Franc Pohleven
Department of Wood Science and Technology, Biotechnical Faculty, University of Ljubljana, 1000 Ljubljana, Slovenia
Borut Strukelj
Department of Pharmaceutical Biology, Faculty of Pharmacy, University of Ljubljana; and Department of Biotechnology, Jozef Stefan Institute, Ljubljana, Slovenia

ABSTRACT

The fruiting body of the culinary-medicinal Maitake mushroom Grifola frondosa contains β-glucans that have immunomodulatory effects and potential uses as adjuncts to cancer therapy. Numerous clinical trials are being conducted to assess the benefits of using commercial preparations containing G. frondosa extracts in cancer treatment. We tested a commercially available product containing polysaccharides from the G. frondosa (CGFP) and studied their effect on gene expression in Jurkat T cells. An mRNA differential display was performed to gain insight into the cell response to the substance. HAI1, hnRNP A2/B1, PKC iota, KLRC1, and genes of the ubiquitin system were identified as possible regulators in the mechanism of tumor-suppressor action of CGFP. Real-time polymerase chain reaction (qPCR) was used to investigate the effect of CGFP on cytokine expression in Jurkat T cells. The qPCR results showed that the expression of the gene for IL-5 is increased.


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