Library Subscription: Guest
Begell Digital Portal Begell Digital Library eBooks Journals References & Proceedings Research Collections
International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v14.i2.20
pages 135-148

Safety Study of Culinary-Medicinal Royal Sun Agaricus, Agaricus brasiliensis S. Wasser et al. KA21 (Higher Basidiomycetes) Assessed by Prokaryotic as well as Eukaryotic Systems

Masuro Motoi
Toei Pharmaceutical Co. Ltd., 2-5-3 Iguchi, Mitaka, Tokyo 181-0011; Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo 192-0392, Japan
Naohito Ohno
Laboratory for Immunopharmacology of Microbial Products, Tokyo University of Pharmacy and Life Science, Tokyo, Japan

ABSTRACT

As part of the safety evaluation of culinary-medicinal Royal Sun Agaricus, Agaricus brasiliensis KA21, for human consumption, we performed the bacterial reverse mutation test, the mouse micronucleus test, and mouse lymphoma test using A. brasiliensis KA21 as the test substance. The reverse mutation test utilized five bacterial strains, including Salmonella typhimurium TA100, TA1535, TA98, and TA1537, and Escherichia coli WP2 uvrAO. For the micronucleus test we used mice. For the mouse lymphoma test, we used one of the most commonly used mammalian cell mutagenesis systems; the L5178YTK +/− mouse lymphoma-TK assay detects the mutations at the thymidine kinase locus caused by base-pair changes, frameshift, and small deletions. All the tests were conducted according to the guidelines for genotoxicity testing of drugs by the Ministry of Health, Labor, and Welfare, Japan. In the bacterial reverse mutation test, no toxicity was observed up to a dose of 5,000 μg/plate. In the mouse micronucleus test, no toxicity was noted up to a dose of 1 g/kg body weight. In the mouse lymphoma test, frequency of the mutation was equal both in the presence or absence of KA21. Supporting the long history of human consumption of A. brasiliensis, the data shown in this study strongly indicate the safety of this mushroom.


Articles with similar content:

Antimicrobial and Antitoxical Action of Edible and Medicinal Mushroom Pleurotus ostreatus (Jacq.: Fr.) Kumm. Extracts
International Journal of Medicinal Mushrooms, Vol.4, 2002, issue 2
Irina V. Tolstych, Olga V. Efremenkova, Valentina A. Zenkova, Olga V. Kamzolkina, Valeriy P. Gerasimenya, Tatyana A. Bogush
Chemopreventive Properties of Mushrooms Against Breast Cancer and Prostate Cancer
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 3
Y.-C. Yuan, K. Lui, J. Ye, G. Hur, D. Smith, S. Chen, S. Phung, S. Kwok, K. Karlsberg, S. Oh
Psathyrella candolleana and Agaricus bisporus Extracts Provide Protection against DNA Oxidative Damage Induced by Doxorubicin
International Journal of Medicinal Mushrooms, Vol.20, 2018, issue 8
Mohammad S. Aladahmy, Mouthana N. Al-Habib, John Holliday
Genotoxicity and Single-Treatment Toxicity Evaluation of Mycoleptodonoides aitchisonii (Agaricomycetes) Water Extract
International Journal of Medicinal Mushrooms, Vol.19, 2017, issue 8
Deuk-Sil Oh, Mi-Ae Bang, Dae-Hun Park, Joung-Wook Seo, Young-Ju Lee, Soon-Young Lee, Ji-Young Kim
Poria cocos (Schwein.) F.A. Wolf in Japanese Traditional Herbal Medicines: Insights from Kampo Case Studies and Implications for Contemporary Research
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 3
Gregory A. Plotnikoff