Library Subscription: Guest
Begell Digital Portal Begell Digital Library eBooks Journals References & Proceedings Research Collections
International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v9.i2.30
pages 123-138

Immunomodulatory and Antitumor Activities of Water-Soluble Proteoglycan Isolated from the Fruiting Bodies of Culinary-Medicinal Oyster Mushroom Pleurotus ostreatus (Jacq.: Fr.) P. Kumm. (Agaricomycetideae)

Saurabh Shah
Department of Biotechnology, Indian Institute of Technology, Kharagpur, India
Dipanjan Ghosh
Department of Biotechnology, Indian Institute of Technology, Kharagpur, India
Sanjaya Kumar Mallick
Department of Biotechnology, Indian Institute of Technology, Kharagpur, India
Itisam Sarangi
Department of Biotechnology, Indian Institute of Technology, Kharagpur, India
Sujit Kumar Bhutia
Department of Biotechnology, Indian Institute of Technology, Kharagpur, India
Indranil Banerje
Department of Biotechnology, Indian Institute of Technology, Kharagpur, India
Swatilekha Maiti
Department of Biotechnology, Indian Institute of Technology, Kharagpur, India
Tapas K Maiti
Department of Biotechnology, Indian Institute of Technology Kharagpur, India

ABSTRACT

Pleurotus ostreatus, also known as oyster mushroom, is studied for its immunomodulatory and anticancer properties. The present study aimed to find a bioactive proteoglycan from a fruiting body of P. ostreatus that could be used as a biological response modifier for immunomodulatory and anticancer effects with the fewest side effects. A water-soluble fraction of alcohol-precipitated fruiting body extract was purified through anion and cation exchange chromatography, followed by gel filtration (Sephadex G-100) chromatography. The purified proteoglycan fraction was represented by the polysaccharide-to-protein ratio of 3.46. The monosaccharide composition analysis of the proteoglycan showed the presence of galactose, mannose, and glucose in the ratio of 12:11:10. The fraction was examined for its immunomodulatory and anticancer effects in a mouse Sarcoma-180 model. The purified proteoglycan stimulated the growth of thymocytes and splenocytes and induced interleukin-4 and interferon gamma in vitro. It also activated macrophages to produce nitric oxide, super oxide anion, and hydrogen peroxide. The growth inhibition of Sarcoma-180 cells both in vivo and in vitro by this proteoglycan fraction was significant and arrested cell cycle in G0/G1 phase.


Articles with similar content:

Effect of Sasa veitchii Extract on Immunostimulating Activity of β-glucan (SCG) from Culinary-Medicinal Mushroom Sparassis crispa Wulf.:Fr. (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.14, 2012, issue 6
Masamichi Tsuboi, Kazuo Takeshita, Masato Kanamori, Yoshiyuki Adachi, Noriko N. Miura, Mia Yoshida, Naohito Ohno, Toshie H. Hida, Natsuko Akachi
Antimicrobial, Cytotoxic, Anti-Inflammatory, and Antioxidant Activity of Culinary Processed Shiitake Medicinal Mushroom (Lentinus edodes, Agaricomycetes) and Its Major Sulfur Sensory-Active Compound−Lenthionine
International Journal of Medicinal Mushrooms, Vol.20, 2018, issue 2
Iveta Stefanova, Roman Kubec, Pavel Hrouzek, Jan Hosek, Zuzana Plavcova, Kristyna Kupcova
Ganoderma lucidum (Curt.: Fr.) P. Karst. (Aphyllophoromycetideae) Inhibits Proliferation of Human Peripheral Blood Lymphocytes by Blocking lnterleukin-2 Secretion
International Journal of Medicinal Mushrooms, Vol.2, 2000, issue 4
Ha Won Kim, Byong Kak Kim, Mi Ja Shim
Immunomodulatory Activities of Different Solvent Extracts from Tricholoma matsutake (S. Ito et S. Imai) Singer (Higher Basidiomycetes) on Normal Mice
International Journal of Medicinal Mushrooms, Vol.14, 2012, issue 6
Xiulian Yin, Qinghong You, Zhonghai Jiang
Immunomodulation Activity of a Polysaccharide Fraction of a Culinary-Medicinal Mushroom, Pleurotus citrinopileatus Singer (Agaricomycetideae), in Vitro
International Journal of Medicinal Mushrooms, Vol.10, 2008, issue 3
Ken-ichiro Minato