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International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v16.i5.30
pages 431-447

Effects of Medium Components and Culture Conditions on Mycelial Biomass and the Production of Bioactive Ingredients in Submerged Culture of Xylaria nigripes (Ascomycetes), a Chinese Medicinal Fungus

Jian-Zhi Chen
Department of Bioindustry Technology, Da-Yeh University, Datsuen, Changhua, Taiwan
Hui-Chen Lo
Department of Nutritional Science, Fu Jen Catholic University, New Taipei City, Taiwan
Fang-Yi Lin
Department of Bioindustry Technology, Da-Yeh University, Datsuen, Changhua, 51591, Taiwan
Shih-Liang Chang
School of Chinese Medicine, China Medical University, Taichung, Taiwan; Department of Medicinal Botanicals and Health Applications, Da-Yeh University, Changhua, Taiwan
Chang-Wei Hsieh
Department of Medicinal Botanicals and Health Applications, Da-Yeh University, Datsuen, Changhua, 51591, Taiwan (R. O. C.)
Zeng-Chin Liang
Department of Bioresources, Da-Yeh University, Changhua County 515, Taiwan
Wai-Jane Ho
Department of Medicinal Botanicals and Healthcare, Department of Bioresources, Da-Yeh University, Datsun, Changhua, 51591 Taiwan
Tai-Hao Hsu
Department of Medicinal Botanicals and Health Applications, Da-Yeh University, Datsuen, Changhua, 51591, Taiwan (R. O. C.); Department of Bioindustry Technology, Da-Yeh University, Datsuen, Changhua, 51591, Taiwan (R. O. C.)

ABSTRACT

The optimal culture conditions were investigated to maximize the production of mycelial biomass and bioactive ingredients in submerged cultivation of Xylaria nigripes, a Chinese medicinal fungus. The one-factor-at-a-time method was used to explore the effects of medium components, including carbon, nitrogen, mineral sources, and initial pH of the medium and environmental factors, such as culture temperature and rotation speed, on mycelial growth and production of bioactive ingredients. The results indicated that the optimal culture temperature and rotation speed were 25°C and 100 rpm in a medium with 20 g fructose, 6 g yeast extract, and 2 g magnesiun sulfate heptahydrate as carbon, nitrogen, and mineral sources, respectively, in 1 L distilled water with an initial medium pH of 5.5. With optimal medium components and conditions of cultivation, the maximal production of mycelial biomass was 6.64 ± 0.88 g/L, with maximal production of bioactive ingredients such as extracellular polysaccharides (2.36 ± 0.18 mg/mL), intracellular polysaccharides (2.38 ± 0.07 mg/g), adenosine (43.27 ± 2.37 mg/g), total polyphenols (36.57 ± 1.36 mg/g), and triterpenoids (31.29 ± 1.17 mg/g) in a shake flask culture. These results suggest that different bioactive ingredients including intracellular polysaccharides, adenosine, total polyphenols and triterpenoids in mycelia and extracellular polysaccharides in broth can be obtained from one simple medium for submerged cultivation of X. nigripes.


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