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International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.433 SNIP: 0.661 CiteScore™: 1.38

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v16.i5.40
pages 449-461

Cloning of Mating-Type Gene MAT1-1 from the Caterpillar Medicinal Mushroom, Cordyceps militaris (Ascomycetes) Using TAIL-PCR Technology

Wei-Ran Cong
Plant Biotechnology Research Center, Key Laboratory of Urban Agriculture (South) Ministry of Agriculture, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, People's Republic of China
Zhen-Hua Gong
Plant Biotechnology Research Center, Key Laboratory of Urban Agriculture (South) Ministry of Agriculture, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, People's Republic of China
Dan-Dan Shi
Plant Biotechnology Research Center, Key Laboratory of Urban Agriculture (South) Ministry of Agriculture, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, People's Republic of China
Hui Guo
Tibet Shenglong Industry Co. Ltd., Lhasa, Tibet, People's Republic of China
Xuan-Wei Zhou
School of Agriculture and Biology and Engineering Research Center of Therapeutic Antibody (Ministry of Education), Shanghai Jiao Tong University, Shanghai, People's Republic of China

ABSTRACT

Cordyceps militaris and Ophiocordyceps sinensis (syn. Cordyceps sinensis), 2 well-known traditional Chinese medicines, contain the same bioactive components and share a similar developmental process. In this study, one C. militaris strain preserved in our laboratory was proven to be a MAT1 mating-type strain using a polymerase chain reaction−based mating-type assay. A 5000-bp nucleotide sequence of the mating-type MAT1-1 from C. militaris was amplified by thermal asymmetric interlaced polymerase chain reaction, but genes within the mating-type MAT1-2 remain undetectable. Sequence analysis shows that the mating-type gene MAT1-1 idiomorph contains 2 genes, MAT1-1-1 and MAT1-1-2. The MAT1-1-1 gene consists of 1480-bp nucleotides that encode 456 amino acids and contain the conserved a-box domain interrupted by 2 introns; the MAT1-1-2 gene consists of 1066 nucleotides that encode 377 amino acids interrupted by one intron. The intervening distance between MAT1-1-1 and MAT1-1-2 is 778 bp. The C. militaris MAT1-1 idiomorph organization is the same as that of Cordyceps takaomontana. The MAT1-1 mating-type idiomorph of both Cordyceps species lacks the MAT1-1-3 gene, which is typically present in Pyrenomycetes. These studies provide some insights for further study of the morphological development of C. militaris and will eventually benefit the domestication of O. sinensis.


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