Library Subscription: Guest
Begell Digital Portal Begell Digital Library eBooks Journals References & Proceedings Research Collections
International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v16.i3.90
pages 293-302

The Optimum Submerged Culture Condition of the Culinary-Medicinal White Jelly Mushroom (Tremellomycetes) and Its Antioxidant Properties

Jessica Krisanti Gusman
Department of Biotechnology, Asia University, Wufeng, Taichung, Taiwan
Chien-Yih Lin
Department of Biotechnology, Asia University, Wufeng, Taichung, Taiwan
Yang-Chia Shih
Department of Biotechnology, Asia University, Wufeng, Taichung, Taiwan

ABSTRACT

Tremella fuciformis is a natural edible and medicinal mushroom commercially available in Taiwan. In this study, the effects of initial pH, incubation time, and various media to optimize exopolysaccharide (EPS) production by T. fuciformis were evaluated in shake flasks and a bioreactor. The antioxidant properties were investigated. The results showed that using potato dextrose broth medium (pH 9) and a 48-hour incubation time in a shake flask was the most efficient condition from which to obtain maximum EPS. However, the quantity of EPS from different initial pH values (5−9) was not significantly different. Total polyphenol and β−glucan contents from every EPS sample (pH 5−9) were evaluated. T. fuciformis polysaccharides (10 mg/mL, pH 9) could scavenge 40.63% of 1,1-diphenyl-2-picrylhydrazyl radicals. The chelating abilities of ferrous ion of all extracts reached more than 80%. EPS extract from the initial pH value of 9 showed the highest reducing power among the other pH values (half maximal effective concentration = 5.97 mg/mL). The EPS from T. fuciformis was noncytotoxic to mouse skin fibroblasts (NIH/3T3), and survival rates were more than 100% using MTT assay. The samples were used to analyze the scavenging activity against oxidative damage induced by ultraviolet B radiation and hydrogen peroxide. The results showed the antioxidant activity occurred in a dose-dependent manner (P < 0.05). Furthermore, these findings prove that the EPS of T. fuciformis from submerged culture possess antioxidant properties and can be used as an alternative treatment for cell protection.


Articles with similar content:

Antioxidant Properties of Polysaccharides from the Willow Bracket Medicinal Mushroom, Phellinus igniarius (L.) Quél. (Aphyllophoromycetideae) in Submerged Culture
International Journal of Medicinal Mushrooms, Vol.11, 2009, issue 4
Ming-Yeou Lung, Jie-Chung Tsai
Mycelial Submerged Culture of New Medicinal Mushroom, Humphreya coffeata (Berk.) Stey. (Aphyllophoromycetideae) for the Production of Valuable Bioactive Metabolites with Cytotoxicity, Genotoxicity, and Antioxidant Activity
International Journal of Medicinal Mushrooms, Vol.11, 2009, issue 4
Benjamin Rojano, Norma A. Valdez-Cruz, Sandra M. Porras-Arboleda, Mauricio Trujillo-Roldan, Leticia Rocha-Zavaleta, Cecilia Aguilar
Antioxidant and Genotoxic Properties of Hispidin Isolated from the Velvet-Top Mushroom, Phaeolus schweinitzii (Agaricomycetes)
International Journal of Medicinal Mushrooms, Vol.19, 2017, issue 11
Veronika Dedonyte, Petras Rimantas Venskutonis, Lina Smolskaite, Gražina Slapšyte, Jurate Mierauskiene
Enhancement of Antioxidant Properties and Increase of Content of Vitamin D2 and Non-volatile Components in Fresh Button Mushroom, Agaricus bisporus (Higher Basidiomycetes) by γ-irradiation
International Journal of Medicinal Mushrooms, Vol.16, 2014, issue 2
Jeng-Leun Mau, Shu-Yao Tsai, Shih-Jeng Huang
Polysaccharides and Antioxidants from Culinary-Medicinal White Button Mushroom, Agaricus bisporus (Agaricomycetes), Waste Biomass
International Journal of Medicinal Mushrooms, Vol.20, 2018, issue 8
Jasenka Piljac-Zegarac, Vlatka Petravić-Tominac, Senka Djakovic, Vesna Zechner-Krpan, Sinisa Srecec, Aleksandra Vojvodic Cebin, Omoanghe S. Isikhuemhen