Published 12 issues per year
ISSN Print: 1521-9437
ISSN Online: 1940-4344
Indexed in
Regulation of Growth and Biosynthetic Activity of the Medicinal Jelly Mushroom Tremella mesenterica (Retz.: Fr.) Pure Culture
ABSTRACT
Different yeast-like budding haploid strains of the yellow brain mushroom Tremella mesenterica (Retz.: Fr.) were obtained using the monobasidiosporous culture method from basidioma specimens collected in Israel. Nutritional requirements for biomass growth and extracellular polysaccharide production were investigated. A two-stage technology for acidic glucuronoxylomannan production was developed. On a first stage inoculum culture medium was balanced for biomass accumulation, and maximal polysaccharide production was obtained on a fermentation medium under conditions of nitrogen limitation. A crude product obtained by alcohol precipitation of culture broth contains 40% of acidic glucuronoxylomannan, 5% of neutral glucuronoxylomannan, 30% of cell biomass, and also includes low molecular weight substances, free amino acids, and mineral elements.