Library Subscription: Guest
Begell Digital Portal Begell Digital Library eBooks Journals References & Proceedings Research Collections
International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.2019031912
pages 909-919

The DnaJ Gene Family in Shiitake Culinary-Medicinal Mushroom, Lentinus edodes (Agaricomycetes): Comprehensive Identification, Characterization, and Expression Profiles under Different Conditions

Gangzheng Wang
Institute of Applied Mycology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China; State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangzhou 510070, China
Yi Luo
Institute of Applied Mycology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China
Wang Chen
Institute of Applied Mycology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China
Yuhua Gong
Institute of Applied Mycology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China
Yan Zhou
Institute of Applied Mycology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China
Yinbing Bian
Institute of Applied Mycology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China


The DnaJ proteins, also called heat-shock protein 40 based on their molecular weight, play significant roles in organism growth and development and resistance to abiotic and biotic stresses. However, studies on the DnaJ gene family in Lentinus edodes (= Lentinula edodes) are less well known. In this study, 29 putative L. edode DnaJ genes (LeDnaJ01 to LeDnaJ29) were identified using bioinformatics analysis and were classified into four groups according to the presence of the J protein and zinc finger as well as C-terminal domain. Multiple cis elements related to the phytohormone and stresses were found in the promotor region of the LeDnaJ genes. In addition, qRT-PCR analysis revealed that 79.31% of LeDnaJ genes were induced by cadmium, 55.17% were induced by Trichoderma atroviride, and 37.93% were induced by heat stress, indicating that the LeDnaJ proteins might participate in the response of L. edodes to the multiple stresses. Meanwhile, qRT-PCR analysis also revealed that all LeDnaJs are expressed in at least one development stage, indicating that they could be involved in the process of L. edodes growth and development and the response to the abiotic and biotic stresses. Taken together, these results advance the functional analysis of DnaJ genes in Basidiomycetes.


  1. Gong WB, Li L, Zhou Y, Bian YB, Kwan HS, Cheung MK, Xiao Y. Detection of quantitative trait loci underlying yield-related traits in shiitake culinary-medicinal mushroom, Lentinus edodes (Agaricomycetes). Int J Med Mushrooms. 2018; 20(5):451-58.

  2. Qiao X, Huang W, Bian Y. Effective removal of cadmium ions from a simulated gastrointestinal fluid by Lentinus edodes. Inter. J Env Res Pub Heal. 2014;11(12):12486-98.

  3. Wang GZ, Cao XT, Ma XL, Guo MP, Liu CH, Yan LL, Bian YB. Diversity and effect of Trichoderma spp. associated with green mold disease on Lentinula edodes in China. Microbiol Open. 2016;5(4):709-18.

  4. Walsh P, Bursac D, Law YC, Cyr D, Lithgow T. The J-protein family: modulating protein assembly, disassembly and trans-location. EMBO Rep. 2004;5(6):567-71.

  5. Li Y, Bu C, Li T, Wang S, Jiang F, Yi Y, Yang H, Zhang Z. Cloning and analysis of DnaJ family members in the silkworm, Bombyx mori. Gene. 2016;576(1):88-98.

  6. Fan F, Yang X, Cheng Y, Kang Y, Chai X. The DnaJ gene family in Pepper (Capsicum annuum L.): comprehensive identification, characterization and expression profiles. Front Plant Sci. 2017;8:689.

  7. Cheetham ME, Caplan AJ. Structure, function and evolution of DnaJ: conservation and adaptation of chaperone function. Cell Stress Chaperon. 1998;3(1):28-36.

  8. Ohtsuka K, Hata M. Mammalian HSP40/DNAJ homologs: cloning of novel cDNAs and a proposal for their classification and nomenclature. Cell Stress Chaperon. 2000;5(2):98-112.

  9. Pulido P, Leister D. Novel DNAJ-related proteins in Arabidopsis thaliana. New Phytol. 2018;217(2):480-90.

  10. Kong F, Deng Y, Wang G, Wang J, Liang X, Meng Q. LeCDJ1, a chloroplast DnaJ protein, facilitates heat tolerance in transgenic tomatoes. J Integr Plant Biol. 2014;56(1):63-74.

  11. Xia Z, Zhang X, Li J, Su X, Liu J. Overexpression of a tobacco J-domain protein enhances drought tolerance in transgenic Arabidopsis. Plant Physiol Bioc. 2014;83:100-6.

  12. Rampuria S, Bag P, Rogan CJ, Sharma A, Gassmann W, Kirti PB. Pathogen-induced AdDjSKI of the wild peanut, Arachis diogoi, potentiates tolerance of multiple stresses in E. coli and tobacco. Plant Sci. 2018;272:62-74.

  13. Xie JL, Bohovych I, Wong EO, Lambert JP, Gingras AC, Khalimonchuk O, Cowen LE, Leach MD. Ydjl governs fungal morphogenesis and stress response, and facilitates mitochondrial protein import via Mas1 and Mas2. Microbial Cell. 2017;4(10):342.

  14. Caplan AJ, Tsai J, Casey PJ, Douglas MG. Farnesylation of YDJ1p is required for function at elevated growth temperatures in Saccharomyces cerevisiae. J Biol Chem. 1998;267(26):18890-95.

  15. Wang GZ, Zhou SS, Luo Y, Ma CJ, Gong YH, Zhou Y, Gao SS, Huang ZC, Hu Y, Bian Y. The heat shock protein 40 LeDnaJ regulates stress resistance and indole-3-acetic acid biosynthesis in Lentinula edodes. Fungal Genet Biol. 2018; 118:37-44.

  16. Wang GZ, Ma CJ, Luo Y, Zhou SS, Zhou Y, Ma XL, Cai YL, Yu JJ, Gong YH. Proteome and transcriptome reveal involvement of heat shock proteins and indoleacetic acid metabolism process in Lentinula edodes thermotolerance. Cell Physiol Biochem. 2018;50(5):1617-37.

  17. Miernyk JA. The J-domain proteins of Arabidopsis thaliana: an unexpectedly large and diverse family of chaperones. Cell Stress Chaperon. 2001;6(3):209.

  18. Sarkar NK, Thapar U, Kundnani P, Panwar P, Grover A. Functional relevance of J-protein family of rice (Oryza sativa). Cell Stress Chaperon. 2013;18(3):321-31.

  19. Chen L, Gong Y, Cai Y, Liu W, Zhou Y, Xiao Y, Xu Z, Liu Y, Lei X, Wang G, Guo M, Ma X, Bian Y. Genome sequence of the edible cultivated mushroom Lentinula edodes (Shiitake) reveals insights into lignocellulose degradation. PloS One. 2016;11(8):e0160336.

  20. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2-AACT method. Methods. 2001;25(4):402-8.

  21. Wang GD, Cai GH, Xu N, Zhang LT, Sun XL, Guan J, Meng QW. Novel DnaJ protein facilitates thermotolerance of transgenic tomatoes. Int J Mol Sci. 2019;20:367.

  22. Georgopoulos CP, Lundquist-Heil A, Yochem J, Feiss M. Identification of the E. coli DnaJ gene product. Mol Gen Genet. 1980;178:583-88.

  23. Osakabe Y, Yamaguchi-Shinozaki K, Shinozaki K, Tran LSP. ABA control of plant macroelement membrane transport systems in response to water deficit and high salinity. New Phytol. 2014;202:35-49.

  24. Sheshadri SA, Nishanth MJ, Simon B. Stress-mediated cis-element transcription factor interactions interconnecting primary and specialized metabolism in planta. Front Plant Sci. 2016;7:1725.

  25. Giarola V, Jung NU, Singh A, Satpathy P, Bartels D. Analysis of pcC13-62 promoters predicts a link between cis-element variations and desiccation tolerance in Linderniaceae. J Exp Bot. 2018;69(15):3773-84.

  26. Lescot M, Dehais P, Thijs G, Marchal K, Moreau Y, Van de Peer Y, Rouze P, Rombauts S. PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences. Nucleic Acids Res. 2002;30(1):325-27.

  27. Bekh-Ochir D, Shimada S, Yamagami A, Kanda S, Ogawa K, Nakazawa M, Matsui M, Sakuta M, Osada H, Asami T, Nakano T. A novel mitochondrial DnaJ/Hsp40 family protein BIL2 promotes plant growth and resistance against environmental stress in brassinosteroid signaling. Planta. 2013;237(6):1509-25.

  28. Zhu X, Liang S, Yin J, Yuan C, Wang J, Li W, He M, Wang J, Chen W, Ma B, Wang Y, Qin P, Li S, Wang Y. The DnaJ Os- DjA7/8 is essential for chloroplast development in rice (Oryza sativa). Gene. 2015;574(1):11-19.

Articles with similar content:

Study of Hypsizygus marmoreus (Peck) Bigel. and Grifola frondosa (Dicks.: Fr.) S.F. Gray: Cultural-Morphological Peculiarities, Growth Characteristics, Qualitative Enzymatic Activity, and Resistance to Fungal Pest Contamination
International Journal of Medicinal Mushrooms, Vol.8, 2006, issue 4
Alexander Beharav, Eden Akavia, Eviatar D. Nevo
Mycelial Growth of Three Pleurotus (Jacq.: Fr.) P. Kumm. Species on Sugarcane Bagasse: Production of Hydrolytic and Oxidative Enzymes
International Journal of Medicinal Mushrooms, Vol.9, 2007, issue 3&4
Dulce Salmones, Erika Gonzalez Cortes, Gerardo Mata
Three Types of Geranylgeranyl Diphosphate Synthases from the Medicinal Caterpillar Fungus, Cordyceps militaris (Ascomycetes)
International Journal of Medicinal Mushrooms, Vol.16, 2014, issue 2
Tao Yang, Caihong Dong, Junde Sun, Tiantian Lian
ITS1/5.8S/ITS2, a Good Marker for Initial Classification of Shiitake Culinary-Medicinal Lentinus edodes (Agaricomycetes) Strains in China
International Journal of Medicinal Mushrooms, Vol.20, 2018, issue 3
Da-Peng Bao, Qi Tan, Ying Song, Yan Zhao, Chuan-Hua Li, Xiao-Xia Song, Chun-Yan Song, Ming-Jie Chen
Human Metallothionein Expression under Normal and Pathological Conditions: Mechanisms of Gene Regulation Based on In silico Promoter Analysis
Critical Reviews™ in Eukaryotic Gene Expression, Vol.19, 2009, issue 4
Pieter De Bleser, Debby Laukens, Claude Cuvelier, Martine De Vos, Anouk Waeytens