Library Subscription: Guest
International Journal of Medicinal Mushrooms

Published 12 issues per year

ISSN Print: 1521-9437

ISSN Online: 1940-4344

The Impact Factor measures the average number of citations received in a particular year by papers published in the journal during the two preceding years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) IF: 1.2 To calculate the five year Impact Factor, citations are counted in 2017 to the previous five years and divided by the source items published in the previous five years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) 5-Year IF: 1.4 The Immediacy Index is the average number of times an article is cited in the year it is published. The journal Immediacy Index indicates how quickly articles in a journal are cited. Immediacy Index: 0.3 The Eigenfactor score, developed by Jevin West and Carl Bergstrom at the University of Washington, is a rating of the total importance of a scientific journal. Journals are rated according to the number of incoming citations, with citations from highly ranked journals weighted to make a larger contribution to the eigenfactor than those from poorly ranked journals. Eigenfactor: 0.00066 The Journal Citation Indicator (JCI) is a single measurement of the field-normalized citation impact of journals in the Web of Science Core Collection across disciplines. The key words here are that the metric is normalized and cross-disciplinary. JCI: 0.34 SJR: 0.274 SNIP: 0.41 CiteScore™:: 2.8 H-Index: 37

Indexed in

Isolation and Regeneration of Protoplasts from Cantharellus cibarius Fr.:Fr., an Edible Ectomycorrhizal Mushroom

Volume 7, Issue 3, 2005, 422 pages
DOI: 10.1615/IntJMedMushrooms.v7.i3.640
Get accessDownload

ABSTRACT

Mushroom mycelia are now considered as a potential source of protein, amino acids, vitamins, and minerals. Commercial production of mushroom mycelia could provide a promising source of proteinaceous food. Cantharellus cibarius is an ectomycorrhizal mushroom, which grows during the rainy season in association with wild bamboo in India in general and in Central India in particular.
The mushroom belongs to the order Cantharellales and the class Basidiomycotina. The mushroom is sold in open markets in the tribal pockets of Melghat (Maharashtra), Tamiya, and Patalkot in District Chhindwara; the Balaghat District of Madhya Pradesh; and the Bastar region of Chhatisgarh states of India. The ectomycorrhizal mushroom C. cibarius was collected from Melghat, Amravati. The gills of this mushroom were used for isolation of the fungus on "Modified-Fries-Medium." Protoplastation is interesting and useful in the case of ectomycorrhizas, in which most of the exchange of solutes takes place in apoplastic space in the hartig net area. These fungi have advantage over arbuscular mycorrhizal fungi because they can be grown on defined synthetic media and thus can be manipulated apart from the plant partner. The isolation of the protoplast can also be used to understand the mystery of symbiosis between the fungal partner and mycelia protoplast can be used to address questions pertaining to metabolic exchange.
Protoplasts of C. cibarius were isolated from mycelium grown in potato-dextrose broth. When mycelia were treated with a combination of macerozyme, chitinase, and cellulase and with 0.6 M sucrose as the osmotic stabilizer, the protoplast yield was 1.0 × 106 per gm fresh weight of mycelium within 3 hours of incubation. The protoplasts were regenerated in solid MFM agar plates. The regeneration frequency was between 20 and 25% and depended on culture conditions.
The main aim of isolation of the protoplast from C. cibarius was to study its regeneration potential.

Begell Digital Portal Begell Digital Library eBooks Journals References & Proceedings Research Collections Prices and Subscription Policies Begell House Contact Us Language English 中文 Русский Português German French Spain