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International Journal of Medicinal Mushrooms
IF: 1.423 5-Year IF: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Print: 1521-9437
ISSN Online: 1940-4344

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v6.i1.70
10 pages

Lignocellulolytic Enzyme Activities of Medicinally Important Basidiomycetes from Different Ecological Niches

Nona A. Mikiashvili
Institute of Evolution, University of Haifa, Israel; Iv. Javakhishvili State University of Tbilisi, Department of Biochemistry and Biotechnology, Georgia; Mushroom Biology & Fungal Biotechnology Laboratory, North Carolina A&T State University, USA
David Chichua
Institute of Biochemistry and Biotechnology, Academy of Science of Georgia, Agmashenebeli Alley 10 km, 380059 Tbilisi, Georgia
Vladimir I. Elisashvili
Animal Husbandry and Feed Production Institute of Agricultural University of Georgia, 240 David Agmashenebeli alley, 0159 Tbilisi, Georgia

ABSTRACT

Screening of 11 species with 26 strains of Basidiomycetes of different ecological niches showed that carboxymethyl cellulase and xylanase levels are low in most investigated species and strains under conditions of submerged fermentation. Trametes hirsutus, 71, Gloeophyllum trabelum, 765, and Trametes versicolor, 145, showed high carboxymethyl cellulase activity (5.65 U/mL, 4.75 U/mL, and 4.29 U/mL), and T. versicolor, 897, was good producer of xylanase (5.54 U/mL). High levels of laccase activity in submerged fermentation of mandarine peels were found in Omphalotus olearius, 174, T. versicolor, 775, and Pleurotus ostreatus, 98 (100.1 nkat/mL, 74.8 nkat/mL, and 62.0 nkat/mL). In solid-state fermentation of mandarine peels, production of laccase was higher, for example in T. versicolor, 145 (104.1 nkat/mL). The highest level of manganese peroxidase activity was in Phellinus robustus, 250 (57.7 nkat/mL). All fungi displayed low levels of all investigated enzyme activity during submerged cultivation in glucosecontaining medium.