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International Journal of Physiology and Pathophysiology
SJR: 0.116

ISSN Print: 2155-014X
ISSN Online: 2155-0158

Archives: Volume 1, 2010 to Volume 9, 2018

International Journal of Physiology and Pathophysiology

DOI: 10.1615/IntJPhysPathophys.v6.i2.30
pages 109-118

Peptide Pro-Gly-Pro Stabilzes Electrophysiological Characteristics of Cultured Hippocampal Neurones during Excitotoxic Damage

Vitally Y. Maslov
Bogomolets Institute of Physiology, NASU, Kyiv, Ukraine
Mycola S. Veselovsky
Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine
Anastasiya A. Moskaluk
Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia
Nikolai F. Myasoedov
Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia
Stanislav I. Shram
Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia
Svetlana A. Fedulova
O. O. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine

ABSTRACT

Effect of culturing with Pro-Gly-Pro peptide on electrophysiological characteristics of hippocampal neurons was studied using glutamate excitotoxicity model (excitotoxic damage was induced by 100 µM glutamate application during 5 min). It has been found that negative changes in neurons cultured with 10 µM Pro-Gly-Pro were less prominent compared to the control ones. In particular, culturing with Pro-Gly-Pro significantly affected the resting potential (-55 ± 4 mV in the control,-29 ± 6 mV after glutamate application, and-38 ± 5 mV after glutamate application at culturing with peptide), action potential amplitude (91 ± 4; 65 ± 5; 84 ± 5 mV), duration (4.3 ± 0.4; 9.5 ± 1.6; 5.2 ± 0.7 ms), depolarization rate (56 [38, 84]; 27 [21, 35]; 46 [28, 62] mV/ms) and repolarization rate (-29 [-38,-27];-20 [-21,-18];-29 [-33,-22] mV/ms). The data obtained suggest that Pro-Gly-Pro peptide exhibits its neuroprotective properties at the level of basic electrophysiological characteristics, although appropriate cellular mechanisms require further investigations.


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