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Plasma Medicine

Published 4 issues per year

ISSN Print: 1947-5764

ISSN Online: 1947-5772

SJR: 0.216 SNIP: 0.263 CiteScore™:: 1.4 H-Index: 24

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Understanding the Differences Between Antimicrobial and Cytotoxic Properties of Plasma Activated Liquids

Volume 8, Issue 3, 2018, pp. 299-320
DOI: 10.1615/PlasmaMed.2018028261
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ABSTRACT

The aqueous environment plays an important role in the transmission of cold plasma effects to both prokaryotic and eukaryotic cells. The exposure of liquids to cold atmospheric plasma discharges results in the generation of secondary reactive species; specifically, hydrogen peroxide (H2O2) seems to be one of the most important amongst the reactive species contained in plasma activated liquids (PALs) in causing cytotoxicity. Detailed understanding of the effects of PALs on cells is essential to harness this new technology. Liquids acting as models for non-complex solutions were generated using a dielectric barrier discharge atmospheric cold plasma (DBD-ACP) system. The chemical characterization of the PAL included its pH and concentrations of hydrogen peroxide, nitrite, and nitrate. The antimicrobial effects of PALs on Gram-positive and Gram-negative bacteria were examined, and cytotoxicity assays were used to elucidate the cytotoxic properties of PALs. The research outcomes showed acidification of plasma activated nonbuffered solutions and differences in concentrations of hydrogen peroxide, nitrite, and nitrate. PALs with different compositions varied in their antibacterial activity and cytotoxic effects, indicating that different reactive species may be responsible for these inactivation processes. Our results suggest that antimicrobial and cytotoxic effects are distinct from each other, which may offer promising approaches for future targeted applications in medicine.

CITED BY
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  2. Ng Sing Wei, Lu Peng, Rulikowska Aleksandra, Boehm Daniela, O'Neill Graham, Bourke Paula, The effect of atmospheric cold plasma treatment on the antigenic properties of bovine milk casein and whey proteins, Food Chemistry, 342, 2021. Crossref

  3. Ng Sing Wei, Tsoukou Evanthia, Chaple Sonal, Boehm Daniela, Bourke Paula, Plasma Treatment of Liquids, in Innovative Food Processing Technologies, 2021. Crossref

  4. Los Agata, Ziuzina Dana, Van Cleynenbreugel Robin, Boehm Daniela, Bourke Paula, Assessing the Biological Safety of Atmospheric Cold Plasma Treated Wheat Using Cell and Insect Models, Foods, 9, 7, 2020. Crossref

  5. Zhao Yi‐Ming, Patange Apurva, Sun Da‐Wen, Tiwari Brijesh, Plasma‐activated water: Physicochemical properties, microbial inactivation mechanisms, factors influencing antimicrobial effectiveness, and applications in the food industry, Comprehensive Reviews in Food Science and Food Safety, 19, 6, 2020. Crossref

  6. Tsoukou Evanthia, Bourke Paula, Boehm Daniela, Temperature Stability and Effectiveness of Plasma-Activated Liquids over an 18 Months Period, Water, 12, 11, 2020. Crossref

  7. He Zhonglei, Charleton Clara, Devine Robert W., Kelada Mark, Walsh John M.D., Conway Gillian E., Gunes Sebnem, Mondala Julie Rose Mae, Tian Furong, Tiwari Brijesh, Kinsella Gemma K., Malone Renee, O'Shea Denis, Devereux Michael, Wang Wenxin, Cullen Patrick J., Stephens John C., Curtin James F., Enhanced pyrazolopyrimidinones cytotoxicity against glioblastoma cells activated by ROS-Generating cold atmospheric plasma, European Journal of Medicinal Chemistry, 224, 2021. Crossref

  8. Tsoukou Evanthia, Delit Maxime, Treint Louise, Bourke Paula, Boehm Daniela, Distinct Chemistries Define the Diverse Biological Effects of Plasma Activated Water Generated with Spark and Glow Plasma Discharges, Applied Sciences, 11, 3, 2021. Crossref

  9. Ng Sing Wei, Slikboer Elmar, Dickenson Aaron, Walsh James L., Lu Peng, Boehm Daniela, Bourke Paula, Characterization of an atmospheric pressure air plasma device under different modes of operation and their impact on the liquid chemistry, Journal of Applied Physics, 129, 12, 2021. Crossref

  10. RASHID M M, RASHID Mamunur, HASAN M M, TALUKDER M R, Rice plant growth and yield: foliar application of plasma activated water, Plasma Science and Technology, 23, 7, 2021. Crossref

  11. Manaloto Eline, Gowen Aoife A., Lesniak Anna, He Zhonglei, Casey Alan, Cullen Patrick J., Curtin James F., Cold atmospheric plasma induces silver nanoparticle uptake, oxidative dissolution and enhanced cytotoxicity in glioblastoma multiforme cells, Archives of Biochemistry and Biophysics, 689, 2020. Crossref

  12. Los Agata, Ziuzina Dana, Boehm Daniela, Cullen Patrick J., Bourke Paula, Dudley Edward G., Inactivation Efficacies and Mechanisms of Gas Plasma and Plasma-Activated Water against Aspergillus flavus Spores and Biofilms: a Comparative Study, Applied and Environmental Microbiology, 86, 9, 2020. Crossref

  13. Song Yuanyuan, Fan Xuetong, Cold plasma enhances the efficacy of aerosolized hydrogen peroxide in reducing populations of Salmonella Typhimurium and Listeria innocua on grape tomatoes, apples, cantaloupe and romaine lettuce, Food Microbiology, 87, 2020. Crossref

  14. Tsoukou Evanthia, Bourke Paula, Boehm Daniela, Efficacy of plasma activated saline in a co-culture infection control model, Scientific Reports, 12, 1, 2022. Crossref

  15. Sampaio Aline da Graça, Chiappim William, Milhan Noala Vicensoto Moreira, Botan Neto Benedito, Pessoa Rodrigo, Koga-Ito Cristiane Yumi, Effect of the pH on the Antibacterial Potential and Cytotoxicity of Different Plasma-Activated Liquids, International Journal of Molecular Sciences, 23, 22, 2022. Crossref

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