Library Subscription: Guest
Plasma Medicine

Published 4 issues per year

ISSN Print: 1947-5764

ISSN Online: 1947-5772

SJR: 0.216 SNIP: 0.263 CiteScore™:: 1.4 H-Index: 24

Indexed in

Atmospheric Pressure Plasma Irradiation on Embryonic Stem Cells: Signals and Differentiation

Volume 7, Issue 3, 2017, pp. 215-225
DOI: 10.1615/PlasmaMed.2017019653
Get accessDownload

ABSTRACT

Mouse embryonic stem cells (ESCs) are pluripotent stem cells (PSCs) derived from pre-implantation embryos. They possess self-renewal properties in vitro and can differentiate into various types of cells. Signaling via extrinsic factors is one of the main factors that determine cell fate during the differentiation of PSCs. For example, fibroblast growth factor (FGF) signal enhances the differentiation of ESCs into ectoderm and neural stem cells in mice and in humans. Recently, reactive oxygen species (ROS) have been reported to regulate various signaling pathways. A large number of reactive species such as ROS and reactive nitrogen species are generated by atmospheric pressure plasma (APP) irradiation. We analyzed the effects of APP irradiation on the maintenance and differentiation of mouse ESCs, focusing on signal control. We found that APP irradiation inhibited the proliferation of mouse ESCs, but did not affect their undifferentiated state. In contrast, APP irradiation did not inhibit the proliferation of embryoid bodies, but did inhibit the differentiation of mouse ESCs to mesoderm and endoderm by inhibiting Wnt signal. APP irradiation enhanced the differentiation to the epiblast, ectoderm, and neuronal lineages by activation of FGF4 signaling. This suggests that the latter effects are caused by the H2O2 generated in the medium by APP irradiation.

CITED BY
  1. Application of Plasma to Humans (Blood Coagulation and Regenerative Medicine), in Plasma Medical Science, 2019. Crossref

  2. Veerana Mayura, Lim Jun-Sup, Choi Eun-Ha, Park Gyungsoon, Aspergillus oryzae spore germination is enhanced by non-thermal atmospheric pressure plasma, Scientific Reports, 9, 1, 2019. Crossref

  3. Przekora Agata, Pawlat Joanna, Terebun Piotr, Duday David, Canal Cristina, Hermans Sophie, Audemar Maïté, Labay Cédric, Thomann Jean-Sébastien, Ginalska Grazyna, The effect of low temperature atmospheric nitrogen plasma on MC3T3-E1 preosteoblast proliferation and differentiation in vitro, Journal of Physics D: Applied Physics, 52, 27, 2019. Crossref

  4. Aman Mohammadi Masoud, Ahangari Hossein, Zabihzadeh Khajavi Maryam, Yousefi Mohammad, Scholtz Vladimír, Hosseini Seyede Marzieh, Inactivation of viruses using nonthermal plasma in viral suspensions and foodstuff: A short review of recent studies, Journal of Food Safety, 41, 5, 2021. Crossref

Begell Digital Portal Begell Digital Library eBooks Journals References & Proceedings Research Collections Prices and Subscription Policies Begell House Contact Us Language English 中文 Русский Português German French Spain