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Plasma Medicine

Published 4 issues per year

ISSN Print: 1947-5764

ISSN Online: 1947-5772

SJR: 0.216 SNIP: 0.263 CiteScore™:: 1.4 H-Index: 24

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Low-Temperature Plasma: An Effective Approach Against Candida albicans Biofilm

Volume 4, Issue 1-4, 2014, pp. 231-244
DOI: 10.1615/PlasmaMed.2014012361
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ABSTRACT

This study evaluated the antifungal potential of low-temperature plasma (LTP) on a 72-hour Candida albicans biofilm. A growth inhibition zone test was conducted with agar plates inoculated with C. albicans and submitted to LTP and argon application at 3 and 10 mm for 10, 30, 60, 90, and 120 seconds. The groups for biofilm assays were 60 seconds of LTP application with a tip-to-sample distance of 3 mm (LTP-3) and 10 mm (LTP-10); −application of only argon gas for 60 seconds with a tip-to-sample distance of 3 mm (Ar-3) and 10 mm (Ar-10); and no treatment. The C. albicans biofilm was grown on saliva-coated discs. The medium was replaced every 24 hours. Confocal laser scanning microscopy revealed the proportion of live and dead cells, and variable pressure scanning electron microscopy (VPSEM) showed biofilm/cell structure. No inhibition zone was observed for control and either Ar groups. For the LTP groups, a progressively increasing of inhibition zone diameter was observed for different treatment durations. The LTP-3 and LTP-10 groups presented higher proportions of dead cells compared with the Ar-3 and Ar-10 groups. VPSEM revealed cell perforations in the LTP-3 and LTP-10 groups. A short period of LTP exposure demonstrated an antifungal effect on C. albicans biofilm.

CITED BY
  1. Jablonowski Lukasz, Matthes Rutger, Duske Kathrin, Kocher Thomas, Plasmaanwendungen in der Zahn-, Mund- und Kieferheilkunde, in Plasmamedizin, 2016. Crossref

  2. Kramer Axel, Matthes Rutger, Bekeschus Sander, Bender Claudia, Napp Matthias, Lademann Olaf, Lademann Jürgen, Weltmann Klaus Dieter, Aktueller und perspektivischer Einsatz kalter Plasmen aus hygienischer Indikation, in Plasmamedizin, 2016. Crossref

  3. Delben Juliana Aparecida, Zago Chaiene Evelin, Tyhovych Natalia, Duarte Simone, Vergani Carlos Eduardo, Seneviratne Chaminda Jayampath, Effect of Atmospheric-Pressure Cold Plasma on Pathogenic Oral Biofilms and In Vitro Reconstituted Oral Epithelium, PLOS ONE, 11, 5, 2016. Crossref

  4. Kramer Axel, Bekeschus Sander, Matthes Rutger, Bender Claudia, Stope Matthias B., Napp Matthias, Lademann Olaf, Lademann Jürgen, Weltmann Klaus-Dieter, Schauer Frieder, Cold Physical Plasmas in the Field of Hygiene-Relevance, Significance, and Future Applications, Plasma Processes and Polymers, 12, 12, 2015. Crossref

  5. Shimizu Tetsuji, Ikehara Yuzuru, Benefits of applying low-temperature plasma treatment to wound care and hemostasis from the viewpoints of physics and pathology, Journal of Physics D: Applied Physics, 50, 50, 2017. Crossref

  6. Application of Plasma to Humans (Blood Coagulation and Regenerative Medicine), in Plasma Medical Science, 2019. Crossref

  7. Figueira Leandro W., Panariello Beatriz H. D., Koga-Ito Cristiane Y., Duarte Simone, Low-Temperature Plasma as an Approach for Inhibiting a Multi-Species Cariogenic Biofilm, Applied Sciences, 11, 2, 2021. Crossref

  8. Strazzi-Sahyon Henrico Badaoui, Suzuki Thaís Yumi Umeda, Lima Glívia Queiroz, Delben Juliana Aparecida, Cadorin Bruno Mena, Nascimento Vanessa do, Duarte Simone, Santos Paulo Henrique dos, In vitro study on how cold plasma affects dentin surface characteristics, Journal of the Mechanical Behavior of Biomedical Materials, 123, 2021. Crossref

  9. Bekeschus Sander, Schmidt Anke, Weltmann Klaus-Dieter, von Woedtke Thomas, The plasma jet kINPen – A powerful tool for wound healing, Clinical Plasma Medicine, 4, 1, 2016. Crossref

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