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Plasma Medicine
SJR: 0.271 SNIP: 0.316 CiteScore™: 1.9

ISSN Print: 1947-5764
ISSN Online: 1947-5772

Plasma Medicine

DOI: 10.1615/PlasmaMed.2014008194
pages 137-152

Experimental Evidences on Synergy of Gas Discharge Agents in Bactericidal Activity of Nonthermal Plasma

Elena V. Sysolyatina
Gamaleya Research Institute of Epidemiology and Microbiology, Ministry of Health and Social Development of Russian Federation, Moscow, Russia
Andrey Mukhachev
Gamaleya Research Institute of Epidemiology and Microbiology, Gamaleya st. 18, Moscow, Russia
Maria Yurova
Gamaleya Research Institute of Epidemiology and Microbiology, Gamaleya st. 18, Moscow, Russia
Mikhail Grushin
Scientific Research Center of Russian Federation TRINITI, Pushkovy st. 12, Troitsk, Moscow region, Russia
Vladimir Karal'nik
Scientific Research Center of Russian Federation TRINITI, Pushkovy st. 12, Troitsk, Moscow region, Russia
Alexander Petryakov
Scientific Research Center of Russian Federation TRINITI, Pushkovy st. 12, Troitsk, Moscow region, Russia
Nikolai Trushkin
Scientific Research Center of Russian Federation TRINITI, Pushkovy st. 12, Troitsk, Moscow region, Russia
Maria Danilova
Gamaleya Research Institute of Epidemiology and Microbiology, Gamaleya st. 18, Moscow, Russia
Boris S. Naroditsky
Gamaleya Research Institute of Epidemiology and Microbiology, Ministry of Health and Social Development of Russian Federation, Moscow, Russia
Alexander L. Gintsburg
Gamaleya Research Institute of Epidemiology and Microbiology, Ministry of Health and Social Development of Russian Federation, Moscow, Russia
Yurii S. Akishev
Scientific Research Center of Russian Federation TRINITI, Pushkovy st. 12, Troitsk, Moscow region, Russia; National Research Nuclear University "MEPhI," Kashirskoe shosse 31, Moscow, 115409, Russia
Svetlana A. Ermolaeva
Gamaleya Research Institute of Epidemiology and Microbiology, Ministry of Health and Social Development of Russian Federation, Moscow, Russia

ABSTRACT

Nonthermal plasmas (NTPs) represent a new class of sterilizing agents. A full set of NTP bioactive factors includes electric components (E, charged particles and electric field), neutral active particles (R), and UV. A specific construction of the direct current (DC) corona source was used that allowed dissection of NTP bioactive factors and quantitative evaluation of their individual and combined action on bacterial pathogens Pseudomonas aeruginosa and Staphylococcus aureus. 10 and 120 s were required for the positive and negative coronas, respectively, to kill 105 colony-forming units (CFU) P. aeruginosa. 10 s was required for both positive and negative coronas to kill 105 CFU S. aureus. For the positive corona, the bactericidal activity of components decreased as R+UV>E+UV>>UV and E+UV>R+UV>>UV for P. aeruginosa and S. aureus, respectively. For the negative corona, the bactericidal activity decreased as R+UV>>E+UV=UV=0 and R+UV>>E+UV>UV~0 for P. aeruginosa and S. aureus, respectively. Despite low, if any, activity of electric components in the negative corona, the whole plasma effect was much higher than the effect of neutral particles and UV alone. The obtained results demonstrated that different combinations of bioactive plasma components exerted diverse species-specific bactericidal effects. It is synergy among plasma bioactive factors that supplies high bactericidal activity of NTP.


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