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Plasma Medicine
SJR: 0.198 SNIP: 0.183 CiteScore™: 0.57

ISSN Print: 1947-5764
ISSN Online: 1947-5772

Plasma Medicine

DOI: 10.1615/PlasmaMed.2017019064
pages 187-200

Bactericidal Effect of a Dielectric Barrier Discharge Plasma Jet Generated in Laminar and Preturbulent Helium Flows

Olga Stepanova
Saint Petersburg State University, 7/9 Universitetskaya nab., St. Petersburg, 199034, Russia
Oksana Rybalchenko
Saint Petersburg State University, 7/9 Universitetskaya nab., St. Petersburg, 199034, Russia
Mikhail Pinchuk
Institute for Electrophysics and Electric Power of Russian Academy of Sciences, 18 Dvortsovaya nab., St. Petersburg, 191186, Russia
Alexander Astafiev
Saint Petersburg State University, 7/9 Universitetskaya nab., St. Petersburg, 199034, Russia
Olga Orlova
Saint Petersburg State University, 7/9 Universitetskaya nab., St. Petersburg, 199034, Russia
Valentin A. Spodobin
Institute for Electrophysics and Electric Power, Russian Academy of Sciences, 18 Dvortsovaya nab., 191186, St.-Petersburg, Russia
Anatoly A. Kudryavtsev
St. Petersburg State University, St. Petersburg, 199034, Russia; ITMO University, 49 Kronverkskiy Ave., St. Petersburg, 197101, Russia

ABSTRACT

The bactericidal effect of a helium dielectric barrier discharge plasma jet generated in laminar (at 3 m/s) and preturbulent (at 10 m/s) helium flows is considered in terms of average discharge power and distance of treatment of Escherichia coli cells grown on a nutrient agar surface in a Petri dish. Sizes of bacteria inhibition zones are estimated and morphological changes in ultrastructure of bacterial cells are revealed using electron microscopy. This study suggests that the gas flow regime is an important factor in the production of the helium plasma jet used to inhibit bacteria. We reveal the different responses of E. coli cells treated by the helium plasma jet generated in laminar and preturbulent gas flows. Numerous focal destructions appear in the cells after 2 min of treatment with the laminar gas flow regime. Along with these focal destructions, expansion of cytoplasm and a superhelix of nucleoids are revealed with the preturbulent regime. We explain differences in behavior of cell morphology under the plasma jet formed at different gas flow regimes by the different gas-dynamic conditions of mixing ambient air into the helium flow.