Published 4 issues per year
ISSN Print: 1947-5764
ISSN Online: 1947-5772
Indexed in
Genotoxic and Cytotoxic Effects of Plasma-Activated Media on Multicellular Tumor Spheroids
Download
ABSTRACT
The purpose of this study was to evaluate the effect of plasma-activated media (PAM) on a multicellular tumor spheroid (MCTS) obtained using HCT116 colon carcinoma cells. PAM was processed by a helium plasma jet during 60−240 seconds of exposure. Spheroids were immersed in PAM at different transfer times (from 15 minutes to 48 hours). The inhibitory effect of PAM on cell growth was first evaluated. This growth inhibition is associated with the regionalized accumulation of DNA damage detected by histone H2AX phosphorylation, leading to massive cell death and loss of the MCTS proliferative region. The use of a hydrogen peroxide (H2O2) scavenger (catalase) in PAM induced the reduction of DNA-damaged cells in MCTS, thus pointing out the role of H2O2 in DNA damage, but growth inhibition was still observed. Finally, whenHCT116 colorectal cancer cells and GM637 human fibroblast cells were immersed in PAM, the expression of γH2AX showed that only HCT116 cells were damaged, emphasizing an interesting selectivity of PAM to inactivate only the cancerous cells in the framework of in vitro cases; this selectivity has already been noted in the literature.
-
Binenbaum Y., Ben-David G., Gil Z., Slutsker Ya. Z., Ryzhkov M. A., Felsteiner J., Krasik Ya. E., Cohen J. T., Yousfi Mohammed, Cold Atmospheric Plasma, Created at the Tip of an Elongated Flexible Capillary Using Low Electric Current, Can Slow the Progression of Melanoma, PLOS ONE, 12, 1, 2017. Crossref
-
Karki Surya B, Gupta Tripti Thapa, Yildirim-Ayan Eda, Eisenmann Kathryn M, Ayan Halim, Investigation of non-thermal plasma effects on lung cancer cells within 3D collagen matrices, Journal of Physics D: Applied Physics, 50, 31, 2017. Crossref
-
Turrini Eleonora, Laurita Romolo, Stancampiano Augusto, Catanzaro Elena, Calcabrini Cinzia, Maffei Francesca, Gherardi Matteo, Colombo Vittorio, Fimognari Carmela, Cold Atmospheric Plasma Induces Apoptosis and Oxidative Stress Pathway Regulation in T-Lymphoblastoid Leukemia Cells, Oxidative Medicine and Cellular Longevity, 2017, 2017. Crossref
-
Boehm Daniela, Bourke Paula, Safety implications of plasma-induced effects in living cells – a review of in vitro and in vivo findings, Biological Chemistry, 400, 1, 2018. Crossref
-
Griseti Elena, Kolosnjaj-Tabi Jelena, Gibot Laure, Fourquaux Isabelle, Rols Marie-Pierre, Yousfi Mohammed, Merbahi Nofel, Golzio Muriel, Pulsed Electric Field Treatment Enhances the Cytotoxicity of Plasma-Activated Liquids in a Three-Dimensional Human Colorectal Cancer Cell Model, Scientific Reports, 9, 1, 2019. Crossref
-
Chauvin Julie, Gibot Laure, Griseti Elena, Golzio Muriel, Rols Marie-Pierre, Merbahi Nofel, Vicendo Patricia, Elucidation of in vitro cellular steps induced by antitumor treatment with plasma-activated medium, Scientific Reports, 9, 1, 2019. Crossref
-
Turrini Eleonora, Laurita Romolo, Simoncelli Emanuele, Stancampiano Augusto, Catanzaro Elena, Calcabrini Cinzia, Carulli Giovanni, Rousseau Martina, Gherardi Matteo, Maffei Francesca, Cocchi Veronica, Lenzi Monia, Pellicioni Valentina, Hrelia Patrizia, Colombo Vittorio, Fimognari Carmela, Plasma‐activated medium as an innovative anticancer strategy: Insight into its cellular and molecular impact on in vitro leukemia cells, Plasma Processes and Polymers, 17, 10, 2020. Crossref
-
Akter Mahmuda, Jangra Anshika, Choi Seung Ah, Choi Eun Ha, Han Ihn, Non-Thermal Atmospheric Pressure Bio-Compatible Plasma Stimulates Apoptosis via p38/MAPK Mechanism in U87 Malignant Glioblastoma, Cancers, 12, 1, 2020. Crossref
-
Vaid A, Patil C, Sanghariyat A, Rane R, Visani A, Mukherjee S, Joseph Alphonsa, Ranjan M, Augustine S, Sooraj KP, Rathore V, Nema SK, Agraj A, Garg G, Sharma A, Sharma M, Pansare K, Krishna CMurali, Banerjee Jyotirmoy, Chandra Sarat, Emerging Advanced Technologies Developed by IPR for Bio Medical Applications ‑.A Review, Neurology India, 68, 1, 2020. Crossref
-
Griseti Elena, Merbahi Nofel, Golzio Muriel, Anti-Cancer Potential of Two Plasma-Activated Liquids: Implication of Long-Lived Reactive Oxygen and Nitrogen Species, Cancers, 12, 3, 2020. Crossref
-
Cosimi J, Merbahi N, Marchal F, Eichwald O, Yousfi M, Active species evolution in the presence of different targets impacted by a helium plasma jet at atmospheric pressure, Journal of Physics D: Applied Physics, 55, 14, 2022. Crossref