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Plasma Medicine

ISSN Print: 1947-5764
ISSN Online: 1947-5772

Plasma Medicine

DOI: 10.1615/PlasmaMed.2017018898
pages 27-41

Cytogenetic and Biochemical Investigations of Cultured Leukemia Cells Exposed to Gliding Arc Discharges

S. A. Montaser
Radiation Biology Department, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority, Cairo, Egypt
M. M. Ahmed
Radiation Biology Department, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority, Cairo, Egypt
Gamal M. El-Aragi
Plasma Department, Nuclear Research Center, Egyptian Atomic Energy Authority, Cairo, Egypt
A. A. Elhadry
Biological Application Department, Nuclear Research Center, Egyptian Atomic Energy Authority, Cairo, Egypt
Z. S. Said
Radiation Safety Department, Nuclear & Radiological Regulatory Authority, Cairo, Egypt

ABSTRACT

One of the most important sources of plasma radiation, gliding arc discharge (GAD), has the potential to combine advantages of both thermal and nonthermal plasmas with destructive physical and biochemical properties. Thus, GAD may be effective when applied to the local microenvironment of leukemia cells, especially in the extracellular matrix (ECM). Blood samples from chronic myelogenous leukemia (CML) patients were studied and compared with matched control samples. Triple blood cultures for each case were exposed to GAD for different time periods (20, 40, and 60 s). Cytogenetic and biochemical parameters were investigated before and after irradiation. We observed significant consequences in ECM structure and cytome assay parameters, such as increased frequencies of dispersal cells, micronuclei, and necrotic and apoptotic cells. In addition, results showed improved values of vascular endothelial growth factor and interleukin-10 and decreased levels of collagen IV. The most effective exposure period was 40 s, which presented highly significant results. This type of energy can stop the progress and development of CML via lyses, cell spreading, and eradication of ECM, especially with the use of GAD for a period of 40 s.