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Critical Reviews™ in Biomedical Engineering
SJR: 0.207 SNIP: 0.376 CiteScore™: 0.79

ISSN Print: 0278-940X
ISSN Online: 1943-619X

Critical Reviews™ in Biomedical Engineering

DOI: 10.1615/CritRevBiomedEng.v28.i12.360
pages 209-211

Microphysiometry to Evaluate Real-Time Response of Mammary Epithelial Cells to IGF-I

Rose Marie Robinson
Department of Chemical Engineering, Virginia Polytechnic Institute and State University
R. Michael Akers
Department of Dairy Science, Virginia Polytechnic Institute and State University
Kimberly E. Forsten
Department of Chemical Engineering. Virginia Polytechnic Institute and State University, 133 Randolph Hall, Blacksburg, VA 24061

ABSTRACT

The CytosensorТ Microphysiometer, a biosensor developed by Molecular Devices, was used to assay rapid binding activity of IGF-I for bovine mammary epithelial cell lines. Insulin-like growth factor-I (IGF-I) is a potent mitogen for mammary epithelial cells and has been implicated in breast cancer cell proliferation, a leading cause of cancer death of women in the U.S. today. IGF-I acts by binding to cell surface receptors. We are interested in how autocrine secretion might alter the activity and regulation of IGF-I.
Real-time changes in excretion of protons, which we assert results from IGF-I binding, are detected by the CytosensorТ Microphysiometer and can be correlated with cellular activity. We present IGF-I dose-dependent responses as well as correlated binding data to detect cell surface receptor concentration and thymidine incorporation results to determine cell proliferation following IGF-I stimulation. We examine the effect of insulin-like binding protein-3 (IGFBP-3) both in the presence and absence of IGF-I. We believe comparison of autocrine and paracrine environments for IGF-I stimulation, and the components contributing to the binding of IGF-I to the cell membrane receptor may provide pertinent information for the development of intervention schemes to slow or interrupt IGF-I binding to tumor cells and therefore cancer growth.


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