Temperature Dependence of the Lactate Dehydrogenase Activity in Tissues of the Black Sea Flatfishes

I. V. Emeretli

doi:10.1615/HydrobJ.v38.i3.130

Hydrobiological Journal

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ISSN Print: 0018-8166

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Temperature Dependence of the Lactate Dehydrogenase Activity in Tissues of the Black Sea Flatfishes

Volume 38, Issue 3, 2002, 8 pages

DOI: 10.1615/HydrobJ.v38.i3.130

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I. V. Emeretli
Institute of Biology of the Southern Seas National Academy of Sciences of Ukraine Sevastopol, Ukraine

ABSTRACT

The activity of lactate dehydrogenase (LDH, EC 1.1.1.27) was studied in spring and autumn at different temperatures of the enzyme incubation in cytoplasm of tissues of two poikilothermic fish species: Scophtalmus maeoticus maeoticus and Platichthys flesus luscus. The enzyme activity decreased in the following series: white muscles - red muscles - gills - gonads - liver; it respectively equaled 0.7631-5.3585, 0.1860-2.0931, 0.0814-0.3946, 0.0338-0.3854, and 0.0035-0.0284 mmole/min·mg. This activity depends on the temperature of the enzyme incubation and the duration of experiments. The LDH activity correlates with the metabolism intensity in tissues, the level of actual fish mobility, the stage of gonad maturation, and temperature peculiarities of the species.

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