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Journal of Environmental Pathology, Toxicology and Oncology

Published 4 issues per year

ISSN Print: 0731-8898

ISSN Online: 2162-6537

The Impact Factor measures the average number of citations received in a particular year by papers published in the journal during the two preceding years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) IF: 2.4 To calculate the five year Impact Factor, citations are counted in 2017 to the previous five years and divided by the source items published in the previous five years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) 5-Year IF: 2.8 The Immediacy Index is the average number of times an article is cited in the year it is published. The journal Immediacy Index indicates how quickly articles in a journal are cited. Immediacy Index: 0.5 The Eigenfactor score, developed by Jevin West and Carl Bergstrom at the University of Washington, is a rating of the total importance of a scientific journal. Journals are rated according to the number of incoming citations, with citations from highly ranked journals weighted to make a larger contribution to the eigenfactor than those from poorly ranked journals. Eigenfactor: 0.00049 The Journal Citation Indicator (JCI) is a single measurement of the field-normalized citation impact of journals in the Web of Science Core Collection across disciplines. The key words here are that the metric is normalized and cross-disciplinary. JCI: 0.59 SJR: 0.429 SNIP: 0.507 CiteScore™:: 3.9 H-Index: 49

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Abrogation of Cell Cycle Checkpoint Controls During Malignant Transformation of Syrian Hamster Embryo Cells is Associated with Decreased Sensitivity to Apoptosis

Volume 20, Issue 3, 2001, 12 pages
DOI: 10.1615/JEnvironPatholToxicolOncol.v20.i3.20
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ABSTRACT

Malachite green (MG), consisting of green crystals with a metallic luster, is highly soluble in water, cytotoxic to various mammalian cells, and may act as a liver tumor promoter. In view of its industrial importance and possible exposure to human beings, MG poses a potential environmental health hazard. We have reported earlier the malignant transformation of Syrian hamster embryo (SHE) cells by MG. In this study, we investigated the effects of MG on flow cytometric cell cycle phase distribution of normal and MG-transformed SHE cells in asynchronous and synchronous cell populations. DNA flow cytometric analysis indicated that culturing cells for 48 hours in a medium containing MG 0.1 [mg/mL induced G2/M arrest in normal cells. Malignant-transformed cells showed no such accumulation of cells at the G2/M phase of the cell cycle in response to MG. Synchronization studies indicated that in the control, both in the presence and absence of MG, cells followed a normal cell cycle pattern up to 16 hours. After 16 hours, in the absence of MG, cells continued a normal cell cycle, whereas in the presence of MG they accumulated at the G2/M phase of the cell cycle. This pattern of accumulation of cells at the G2/M checkpoint control was not observed in either untreated or MG-treated transformed cells. We also studied the effects of MG on the induction of apoptosis using flow cytometric FSC/SSC scatter plots in normal and transformed SHE cells. Flow cytometric analysis showed a dose- and time-dependent induction of apoptosis by MG in control cells, whereas induction of apoptosis by MG was marginal in transformed cells. In the present study, we demonstrated the efficient operation of the G2/M checkpoint control, apoptosis in control SHE cells, the abrogation of checkpoint controls, and decreased sensitivity to apoptosis in transformed SHE cells.

CITED BY
  1. Pierrard Marie-Aline, Kestemont Patrick, Delaive Edouard, Dieu Marc, Raes Martine, Silvestre Frédéric, Malachite green toxicity assessed on Asian catfish primary cultures of peripheral blood mononuclear cells by a proteomic analysis, Aquatic Toxicology, 114-115, 2012. Crossref

  2. Hurley Killian, Lacey Noreen, O’Dwyer Ciara A., Bergin David A., McElvaney Oliver J., O’Brien M. Emmet, McElvaney Oisín F., Reeves Emer P., McElvaney Noel G., Alpha-1 Antitrypsin Augmentation Therapy Corrects Accelerated Neutrophil Apoptosis in Deficient Individuals, The Journal of Immunology, 193, 8, 2014. Crossref

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