Begell House Inc.
Plasma Medicine
PMED
1947-5764
3
4
2013
Promising Trial for Treatment of Chronic Myelogenous Leukemia Using Plasma Technology
243-265
10.1615/PlasmaMed.2014011882
M. M.
Ahmed
Radiation Biology Department, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority, Cairo, Egypt, 11787
Gamal M.
El-Aragi
Plasma Physics and Nuclear Fusion Department, Nuclear Research Center (NCR), Egyptian Atomic Energy Authority, Cairo, Egypt, 11787
Abdel Monsef A.
Elhadary
Biological Application Dept., Nuclear Research center, Atomic Energy Authority Cairo, Egypt
Z. S.
Said
Radiation Safety Department, Nuclear
& Radiological Regulatory Authority, Cairo, Egypt
plasma jet
cytokinesis block micronucleus assay
chronic myelogenous leukemia
TGF-B1
arginase
This article presents a trial of the treatment of chronic-phase (CP) and accelerated-phase (AP) chronic myelogenous leukemia (CML) that is resistant or only partially responded to chemotherapy. Blood samples of 6 cases diagnosed with CML were studied and compared with a control group. The first 3 cases were AP CML resistant to imatinib and nilotinib. The other 3 partially responded to the chemotherapy and returned to CP CML. Triple blood cultures for each case were exposed to a cold, pulsed, atmospheric pressure plasma jet for different durations (40, 80, and 120 seconds). Hematological, cytogenetic, and biochemical investigations were done before and after plasma jet exposure. The results showed an increase in necrotic and apoptotic cell counts and a decrease in the number of characteristic nucleoplasmic bridges (multinucleated threadlike shape). Concentrations of transforming growth factor-β1 and arginase decreased in the CML blood samples after exposure to plasma jet.This type of nonthermal plasma can kill cancer cells and prevent the cells from dividing, especially for the 80-second duration.
Cold Physical Plasma Treatment Alters Redox Balance in Human Immune Cells
267-278
10.1615/PlasmaMed.2014011972
Sander
Bekeschus
Leibniz-Institute for Plasma Science and
Technology (INP Greifswald), ZIK Plasmatis, Greifswald, Germany
Thomas
von Woedtke
Leibniz Institute for Plasma Science and Technology e.V. (INP), Greifswald, Germany
Axel
Kramer
Institute of Hygiene and Environmental Medicine,
University Medicine Greifswald, 17475 Greifswald, Germany
Klaus-Dieter
Weltmann
Leibniz-Institute for Plasma Science and
Technology (INP Greifswald), ZIK Plasmatis, Greifswald, Germany
Kai
Masur
Center for Innovation Competence plasmatis, Greifswald, Germany; Leibniz Institute for Plasma Science and Technology, Greifswald, Germany
apoptosis; cold atmospheric pressure plasma; oxidative stress; plasma medicine; redox balance
Cold atmospheric pressure plasma is a promising tool for various biomedical applications. Particularly, treatment of cells and tissues in diseases such as chronic wounds possesses high potential. However, detailed knowledge of how plasma mediates its actions on cells is necessary to explore its potentially beneficial effects in clinical settings. Previous studies have shown that plasma induces oxidative stress. We confirmed this hypothesis by showing that plasma significantly oxidized glutathione (GSH), a major cellular reductant. In plasma-treated cells we
found elevated levels of GSH, pointing to a change in cellular redox balance. Oxidative stress can induce apoptosis and plasma-mediated apoptosis has been shown before measuring phosphatidylserine exposure. Using primary human immune cells, we investigated what events precede this reaction. Apoptosis is an active cellular process and accordingly it was dependent on incubation
temperature after treatment. Damage of mitochondria was linked to apoptosis previously and plasma treatment resulted in mitochondrial oxidation and reduced mitochondrial membrane potential. Further, we measured a treatment time dependent activation of executioner caspase 3 which is known to be crucially involved in apoptosis. Together, our results suggested that plasma-mediated oxidative stress reactions in eukaryotic cells are in line with the foregoing research in redox biology. Establishing this link will help anticipate results in future research and clinical studies involving cold atmospheric pressure plasmas.
Characterization of Plasma Parameters and Tissue Injury Produced by Plasma Electrosurgical Systems
279-289
10.1615/PlasmaMed.2014011979
Jerome
Canady
Jerome Canady Research Institute for Advanced Biological and Technological Sciences, US Medical innovation LLC, Takoma Park, MD, USA
Alexey
Shashurin
The George Washington University; School of Aeronautics and Astronautics, Purdue University, West Lafayette, IN, USA
K.
Wiley
Jerome Canady Research Institute for Advanced Biological and Technological Sciences, 6930 Carroll Avenue, Suite 300, Takoma Park, MD 20912
Nathaniel J.
Fisch
Department of Astrophysical Sciences, Princeton University, Princeton, NJ 08543
Michael
Keidar
Department of Mechanical and Aerospace Engineering, The George Washington University, Science and Engineering Hall, 800 22nd Street, NW, Room 3550, Washington, DC 20052, USA
Plasma medicine
electrosurgery
Plasma and injury properties produced by US Medical Innovations (USMI) electrosurgical systems were characterized using an explant pig's liver samples. It was observed that plasma length, tissue temperature and injury size increases with applied power increase. Transition from Conventional to Argon Coagulation mode (<0.5 L/min) leads to redistribution of the discharge power over the larger tissue area causing abrupt decrease of injury depth and increase of eschar diameter. Flow rate is not a primary factor affecting the tissue temperature. The depth and diameter of injury was minimal for the case of Hybrid Argon Plasma Cut operational mode.
Nonthermal Plasma-Assisted Trauma Management: Hemostasis of Noncompressible Profuse Hemorrhage
291-314
10.1615/PlasmaMed.2014012024
Atharva
Amritkar
Electrical and Computer Engineering, Rowan University, Glassboro, New Jersey, USA
Becky
Cunningham
Mechanical Engineering, Rowan University, Glassboro, New Jersey, USA
Bradly
Hawkins
Chemical Engineering, Rowan University, Glassboro, New Jersey, USA
Brennan
Batalla
Electrical and Computer Engineering, Rowan University, Glassboro, New Jersey, USA
David
Moore
Mechanical Engineering, Rowan University, Glassboro, New Jersey, USA
Eric
Thompson
Mechanical Engineering, Rowan University, Glassboro, New Jersey, USA
Matt
Rossett
Mechanical Engineering, Rowan University, Glassboro, New Jersey, USA
Rittick
Gupta
Electrical and Computer Engineering, Rowan University, Glassboro, New Jersey, USA
Justine
Han
Chemical and Biological Engineering Department, Drexel University 3141 Chestnut Street, Philadelphia, PA 19104
Justin
Johnson
Health Onvector Inc.
nonthermal plasma; plasma medicine; hemostasis; coagulation; blood; hemorrhage; trauma
In this manuscript we discuss chemical kinetic model of blood coagulation by treatment with non-equilibrium atmospheric pressure dielectric barrier discharge. We then add kinetic data into a computational fluid dynamics model of blood flow treated by plasma and show that we are able to induce hemostasis. We then present initial experimental validation of these computational models using in vitro bovine blood samples.
Nonequilibrium Atmospheric Pressure Forward-Vortex Plasma System for Generation of Reactive Species in Flowing Water for Medical Applications
315-332
10.1615/PlasmaMed.2014012074
Tony
Liang
Mechanical Engineering and Mechanics, Drexel University, Philadelphia, Pennsylvania, USA
Justine
Han
Chemical and Biological Engineering Department, Drexel University 3141 Chestnut Street, Philadelphia, PA 19104
Selli
Abdali
Biological Sciences, College of Arts and Sciences, Drexel University, Philadelphia, Pennsylvania, USA
Justin
Ruegg
Advanced Plasma Solutions, Malvern, Pennsylvania; Mechanical Engineering, Rowan University, Glassboro, New Jersey, USA
Alexander
Rabinovich
Nyheim Plasma Institute, Drexel University, Camden, NJ 08103, USA
plasmatron
gliding arc
forward vortex
water sterilization
water cleaning
Recent research in plasma applications in bioengineering and plasma medicine gives attention to plasma treatment of various liquids, especially water, for the growing number
of medical and biological applications. In this paper we present a forward-vortex nonequilibrium atmospheric pressure plasma system for generation of nitrogen and oxygen reactive species as well as reduced pH in flowing water. We discuss the dependence of pH and nitrate produced in water on both the carrier gas and the gas feed rate.
INDEX to Volume 3
333-338
10.1615/PlasmaMed.v3.i4.60