Begell House Inc.
International Journal of Medicinal Mushrooms
IJM
1521-9437
22
7
2020
Cordycipitoid Fungi Powders Promote Mycelial Growth and Bioactive-Metabolite Production in Liquid Cultures of the Stout Camphor Medicinal Mushroom Taiwanofungus camphoratus (Agaricomycetes)
615-626
10.1615/IntJMedMushrooms.2020035440
Yao
Wang
Yunnan Herbal Laboratory, Institute of Herb Biotic Resources, Yunnan University, Kunming 650091, P.R. China; The International Joint Research Center for Sustainable Utilization of Cordyceps Bioresources in China and Southeast Asia, Yunnan University, Kunming 650091, P.R. China
Yan-Fang
Liu
Yunnan Herbal Laboratory, Institute of Herb Biotic Resources, Yunnan University, Kunming 650091, P.R. China; The International Joint Research Center for Sustainable Utilization of Cordyceps Bioresources in China and Southeast Asia, Yunnan University, Kunming 650091, P.R. China
Qi
Fan
Yunnan Herbal Laboratory, Institute of Herb Biotic Resources, Yunnan University, Kunming 650091, P.R. China; The International Joint Research Center for Sustainable Utilization of Cordyceps Bioresources in China and Southeast Asia, Yunnan University, Kunming 650091, P.R. China
Yuan-Bing
Wang
Yunnan Herbal Laboratory, Institute of Herb Biotic Resources, Yunnan University, Kunming 650091, P.R. China; The International Joint Research Center for Sustainable Utilization of Cordyceps Bioresources in China and Southeast Asia, Yunnan University, Kunming 650091, P.R. China; The Research Center of Cordyceps Development and Utilization of Kunming, Yunnan Herbal Biotech Co. Ltd., Kunming 650106, P.R. China
Hong
Yu
Yunnan Herbal Laboratory, Institute of Herb Biotic Resources, Yunnan University, Kunming 650091, P.R. China; The International Joint Research Center for Sustainable Utilization of Cordyceps Bioresources in China and Southeast Asia, Yunnan University, Kunming 650091, P.R. China
cordycipitoid fungi
liquid fermentation
response surface methodology
Taiwanofungus camphoratus
medicinal mushrooms
Taiwanofungus camphoratus, a medicinal mushroom indigenous to Taiwan, has attracted the attention of pharmaceutical companies due to its remarkable properties and considerable commercial value. Since it grows slowly and most of its components are chemically unstable, its production and distribution have been problematic. In the present study, cordycipitoid fungi powders of seven species were used to cultivate T. camphoratus, and optimal conditions for biomass production were determined by response surface methodology. The initial liquid medium was enriched with cordycipitoid fungi powders, inoculated with a spore suspension of T. camphoratus, and then incubated on a rotary shaker (120 r/min at 27°C) for 14 days. The effects of cordycipitoid fungi powders on T. camphoratus production were investigated by examining the biomass production of T. camphoratus. The key factors influencing biomass production, as identified by a two-level Plackett-Burman design with eight variables, were (1) powder content of cordycipitoid fungi, (2) glucose content, and (3) bottling volume. Box-Behnken design and response surface analysis were applied to further investigate the mutual interactions among these factors, and to obtain optimal values leading to maximal biomass yields. Levels of triterpenoids, polysaccharides, mannitol, adenosine, and ergosterol were determined as estimates of the medicinal value of T. camphoratus cultured with or without cordycipitoid fungi powders. The results showed that Paecilomyces hepiali, Metacordyceps neogunnii, and Beauveria bassiana promoted mycelial growth of T. camphoratus, with P. hepiali showing the most prominent effect. The optimal conditions promoting maximal biomass production were found to be as follows: 6.93 g/L P. hepiali powder content; 26.48 g/L glucose content; 180.55 mL of bottling volume (in a 500-mL Erlenmayer flask). Under these conditions, the biomass production was increased by 38.32%, from 13.10 to 18.12 g/L. The polysaccharide, mannitol, adenosine and ergosterol contents, but not the triterpenoid contents of T. camphoratus cultured with P. hepiali powder, were noticeably higher than when cultured with no powder (control condition), and were higher than those of the P. hepiali powder itself. These results indicate the feasibility of large-scale fermentation of T. camphoratus to produce valuable substances that may be used in pharmaceutical products.
Comparative Study on Bioactivities from Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), Gives an Insight into the Fermentation Broth Showing Greater Antioxidative Activities
627-639
10.1615/IntJMedMushrooms.2020035042
Chunliang
Xie
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, P.R. China
Pingping
Tang
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, P.R. China; Key Laboratory of Protein Chemistry and Developmental Biology of Education Committee, College of Life Sciences, Hunan Normal University, Changsha, P.R. China
Shaowei
Yan
Hunan Yujia Cosmetics Manufacturing Co. Ltd., No. 668 Qingshan Road, High-Tech Development Zone, Changsha, P.R. China
Qi
Yang
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, P.R. China
Zhoumei
Zhang
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, P.R. China
Wenbing
Gong
Institute of Applied Mycology, Huazhong Agricultural University, Wuhan, People's Republic of China; Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, People's Republic of China
Zuohua
Zhu
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, P.R. China
Yingjun
Zhou
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, P.R. China
Li
Yan
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, P.R. China
Zhenxiu
Hu
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, P.R. China
Xianchun
Wang
Key Laboratory of Protein Chemistry and Developmental Biology of Education Committee, College of Life Sciences, Hunan Normal University, Changsha, P.R. China
Yuande
Peng
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, P.R. China
Ganoderma lucidum
antioxidation
extract
fermentation broth
tyrosinase inhibition
medicinal mushrooms
Ganoderma lucidum is one of the most famous mushrooms in traditional Chinese medicine. At present, the fully utilized parts of G. lucidum are mainly fruiting body and spore powder. The wild and artificially cultivated G. lucidum fruiting body is costly and rare. Therefore, how to improve the utilization of G. lucidum by means of fermentation is worth investigating. The present study was to perform submerged fermentation of G. lucidum and compare the bioactivities of G. lucidum submerged fermentation broth and fruiting body extract. After the extraction and submerged fermentation methods were optimized, the optimum conditions for extraction were determined as ethanol extraction at 80°C with a solid-to-liquid ratio of 1:30, and those for submerged fermentation were cultivation on malt extract medium for 6 days at 30°C. Under the optimum conditions, the antioxidative activity and tyrosinase inhibition rate of the fermentation broth were 1.2-4.1 fold higher than those of the ethanol extract. Cytotoxicity analysis showed that the ethanol and water extracts and the fermentation broth effectively inhibited pancreatic cancer cells and prostate cancer cells, with much smaller effect on nontumor human embryonic kidney (HEK293T). These results demonstrate that the submerged fermentation could improve the utilization value of G. lucidum and the fermentation broth can be used as an antioxidant additive applied in food, drugs, and cosmetics.
In Vitro Antioxidant Efficacy of Some Selected Medicinal Mushrooms from India
641-649
10.1615/IntJMedMushrooms.2020035358
Uzma
Azeem
Government College Malerkotla, Malerkotla–148023, Punjab, India
Richa
Shri
Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala-147002, Punjab, India
Gurpaul Singh
Dhingra
Department of Botany, Punjabi University, Patiala, Punjab, India
antioxidants
Inonotus
oxidative stress
Phellinus species
phenols
medicinal mushrooms
Oxidative stress is strongly implicated in aging and in the progression of diseases. Antioxidants, especially of natural origin, are valued for their protective as well as curative health benefits. Numerous mushroom species have shown marked antioxidant effect. This can prove beneficial when mushrooms are used as nutraceuticals or for drug development. In the current investigation the antioxidant potential of some medicinal mushrooms, namely Inonotus pachyphloeus, Phellinus allardii, Ph. fastuosus, Ph. sanfordii and Ph. torulosus was examined employing different assays. The ethanol extracts of the fruiting bodies of these mushrooms were evaluated in vitro for scavenging potential against DPPH, hydroxyl radicals, superoxide radicals, as well as the reducing power. The free radical scavenging activity of mushroom extracts followed the trend of Ph. fastuosus > Ph. sanfordii > Inonotus pachyphloeus > Ph. torulosus > Ph. allardii. Ph. fastuosus and Ph. sanfordii extracts exhibited significant DPPH and superoxide radical scavenging activities, statistically (P < 0.05) comparable to each other and to the standard catechin. Ph. fastuosus extract (EC50 = 16 ± 1.15 μ;g/mL) showed the most significant hydroxyl radical scavenging activity even higher than the standard. In reducing power assay, Ph. torulosus extract (EC50 = 320 ± 0.02 μ;g/mL) exhibited the most significant reducing power statistically (P < 0.05) comparable with the reduced form of glutathione. The tested mushroom extracts were found to consist of appreciable amounts of carbohydrates, phenols and proteins. The free radical scavenging efficacy of the examined mushrooms showed positive correlation with their phenol content. These medicinal mushrooms are good natural antioxidants and can be incorporated in nutraceuticals/pharmaceuticals after detailed studies.
Dietary Fiber Contents and Its Fermentability In Vitro of Pleurotus ostreatus cv. Florida Mycelia (Agaricomycetes)
651-657
10.1615/IntJMedMushrooms.2020035449
Janice C.
Laforteza
Graduate School, University of Santo Tomas, España, Manila, Philippines; Central Luzon State University, Science City of Muñoz, Nueva Ecija, Philippines
Renato G.
Reyes
Center for Tropical Mushroom Research and Development, Central Luzon State University, Science City of Munoz, Nueva Ecija, Philippines;Department of Biological Sciences, College of Arts and Sciences, Central Luzon State University, Science City of Muñoz, Nueva Ecija, Philippines
Trinidad P.
Trinidad
Graduate School, University of Santo Tomas, España, Manila, Philippines
Pleurotus ostreatus cv. Florida
mycelia
dietary fibers
fermentability in vitro
medicinal mushrooms
Oyster mushrooms (Pleurotus spp.) constitute a substantial resource for functional food, as they are commercially cultivated worldwide and assumed to contain beneficial fiber. The objective of this study was to characterize and analyze the mycelia of Pleurotus ostreatus cv. Florida for its nutrient composition, total dietary fiber, insoluble dietary fiber, soluble dietary fiber content, and fermentability in vitro. Findings showed the mycelia had a moisture content of 18%, ash 12%, protein 5%, fat 0.1%, and carbohydrates 61%. Results also revealed that mycelia are an excellent source of dietary fiber (55%) containing high amounts of insoluble (22%) and soluble (33%) fiber. The mycelia also produced significant amounts of short chain fatty acids after fermentation at in vitro simulating conditions in the colon: acetate (1.92 mg/g), propionate (0.89 mg/g), and butyrate (0.31 mg/g). These results indicated that the mycelia of P. ostreatus cv. Florida are promising sources of dietary fiber.
Screening of Georgian Medicinal Mushrooms for Their Antibacterial Activity and Optimization of Cultivation Conditions for the Split Gill Medicinal Mushroom, Schizophyllum commune BCC64 (Agaricomycetes)
659-669
10.1615/IntJMedMushrooms.2020035051
Tamar
Khardziani
Durmishidze Institute of Biochemistry and Biotechnology, Academy of Science of Georgia, 10 km Agmashenebeli kheivani, 0159 Tbilisi, Georgia; Institute of Microbial Biotechnology, Agricultural University of Georgia, Tbilisi, Georgia
Eka
Metreveli
The Institute of Microbial Biotechnology, Agricultural University of Georgia, 0131 Tbilisi, Georgia
Kakha
Didebulidze
Institute of Microbial Biotechnology, Agricultural University of Georgia, Tbilisi, Georgia
Vladimir I.
Elisashvili
The Institute of Microbial Biotechnology, Agricultural University of Georgia, 0131 Tbilisi, Georgia
antibacterial activity
medicinal mushrooms
Schizophyllum commune
submerged cultivation
The present study aimed to evaluate the antibacterial activity (ABA) of new mushroom strains collected from the mountain and plain forests of Georgia and belonging to different taxonomic groups. Of 30 Basidiomycetes strains tested on agar plates, Schizophyllum commune BCC64 exhibited the highest inhibitory activity against Escherichia coli and Staphylococcus aureus by the diameter of inhibition zones (17 ± 1 mm and 19 ± 1 mm, respectively). Moreover, this mushroom showed strong activity against Staphylococcus enteritidis (11 mm), Pseudomonas aeruginosa (19 mm), and Salmonella epidermitidis (12 mm). In the submerged cultivation in synthetic medium, xylose and glucose ensured the highest ABA toward S. aureus (70% inhibition in microplate rider tests) and E. coli (60%), respectively. Among lignocellulosic materials tested in the submerged and solid-state fermentation, mandarin marc was found to be an excellent growth substrate for ABA accumulation by Sch. commune 64. Of six nitrogen sources, KNO3 favored the mushroom ABA increase against both bacteria. The suitability of the developed nutrient medium has been proven in 7 L fermenter. After fermentation, ethyl acetate extract obtained from culture liquid and ethanol extract obtained from mycelial biomass of Sch. commune 64 showed the best minimum inhibitory concentration (MIC) against E. coli (0.5 and 2.5 mg/mL, respectively) and S. aureus (1 mg/mL for both extracts).
Determination of Tissue Protective and Antioxidant Activities of Two Medicinal Mushrooms from Turkey against CCl4-lnduced Experimental Oxidative Stress in Rats
671-681
10.1615/IntJMedMushrooms.2020035054
Songul
Hasar
Department of Biology, Institute of Natural and Applied Sciences, Van Yuzuncu Yil University, Van, Turkey
Abdulahad
Dogan
Department of Biochemistry, Faculty of Pharmacy, Van Yuzuncu Yil University, Van, Turkey
Kenan
Demirel
Department of Biology, Faculty of Science, Van Yuzuncu Yil University, Van, Turkey
Agrocybe cylindracea
Lactarius deliciosus
antioxidant defense system
CCl4
lipid peroxidation
oxidative stress
serum enzymes
rat
medicinal mushrooms
This study aimed at determining hepatoprotective and antioxidants effects of Lactarius deliciosus and Agrocybe cylindracea against CCl4-induced oxidative stress (OS) in rats. Herein, 36 rats were divided into 6 groups: (I) Control, (II) CCl4, (III) CCl4 + L. deliciosus, (IV) L. deliciosus, (V) CCl4 + A. cylindracea, and (VI) A. cylindracea. According to the 7-week study results, aspartate aminotransferase (AST), alanine aminotransferase (ALT), glutathione S-transferase (GST) (brain), superoxide dismutase (SOD) (kidney), malondialdehyde (MDA) (kidney), MDA (spleen), MDA (erythrocyte), GST (erythrocyte), and GST (liver) in group III (at 10%, 26%, 34%, 57%, 7%, 25%, 42%, 27%, and 35%, respectively) were significantly lower than in group II. Moreover, the reduced glutathione (GSH) (spleen) and catalase (spleen) in group III (at 20% and 38%, respectively) were significantly higher than in group II. AST, ALT, triglyceride, glutathione reductase (GR) (brain), GR (kidney), SOD (spleen), and SOD (liver) in group V (at 194%, 147%, 38%, 36%, 66%, 8%, and 7%, respectively) were significantly higher than in group II. However, MDA (kidney), MDA (erythrocyte), (GSH) (erythrocyte), MDA (liver), GSH (liver), and GST (liver) in group V (at 48%, 35%, 34%, 15%, 30%, and 41%, respectively) were significantly lower than in group II. Hence, while L. deliciosus may exhibit tissue-protective against CCl4-induced OS in rats, A. cylindracea does not.
Pharmacological Investigation of Ceraceomyces tessulatus (Agaricomycetes) in Mice with Nonalcoholic Steatohepatitis
683-692
10.1615/IntJMedMushrooms.2020035048
Kenichi
Watanabe
Department of Clinical Pharmacology, Faculty of Pharmaceutical Sciences, Niigata University and Pharmacy and Applied Life Sciences, Akiha-ku, Niigata 956-8603, Japan; Department of Laboratory Medicine and Clinical Epidemiology for Prevention of Noncommunicable Diseases, Niigata University Graduate School of Medical and Dental Sciences, Chuo-ku, Niigata 951-8510, Japan
Rejina
Afrin
Department of Clinical Pharmacology, Faculty of Pharmaceutical Sciences, Niigata University of Pharmacy and Applied Life Sciences, Niigata 956-8603, Japan; Department of Pharmacy, East West University, Dhaka-1212, Bangladesh
Remya
Sreedhar
Department of Clinical Pharmacology, Faculty of Pharmaceutical Sciences, Niigata University of Pharmacy and Applied Life Sciences, Niigata 956-8603, Japan
Vengadeshprabhu
Karuppagounder
Department of Clinical Pharmacology, Faculty of Pharmaceutical Sciences, Niigata University of Pharmacy and Applied Life Sciences, Niigata 956-8603, Japan; Department of Orthopedics and Rehabilitation, Penn State College of Medicine, 500 University Drive, Hershey, PA 17033, USA
Meilei
Harima
Department of Clinical Pharmacology, Faculty of Pharmaceutical Sciences, Niigata University of Pharmacy and Applied Life Sciences, Niigata 956-8603, Japan; Niigata University of Rehabilitation, Faculty of Allied Health Sciences, 2-16, Kaminoyama, Murakami, Niigata
958-8292, Japan
Xavier
Alexander
Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research, 168 Manicktala Main Road, Kolkata 700 054, West Bengal, India
Ravichandiran
Velayutham
Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research, 168 Manicktala Main Road, Kolkata 700 054, West Bengal, India
Somasundaram
Arumugam
Department of Clinical Pharmacology, Faculty of Pharmaceutical Sciences, Niigata University of Pharmacy and Applied Life Sciences, Niigata 956-8603, Japan; Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research, 168 Manicktala Main Road, Kolkata 700 054, West Bengal, India
Ceraceomyces tessulatus
fibrosis
SIRT1
inflammation
nonalcoholic steatohepatitis
medicinal mushrooms and fungi
Nonalcoholic steatohepatitis (NASH) is becoming the most common cause of hepatocellular carcinoma (HCC). Natural products including edible mushrooms are gaining attention for the prevention and treatment of lifestyle related disorders. Ceraceomyces tessulatus (strain BDM-X) possesses potent antioxidative stress activity. In this study, we hypothesize that BDM-X treatment protects the liver of mouse with NASH by reducing inflammation in a novel NASH-HCC mouse model. C57BL/6J female pups were exposed to low-dose streptozotocin (STZ) and fed a high-fat diet (HFD) 32 from the age of 4 weeks to 16 weeks. Water extract of BDM-X was given at 500 mg/kg dose daily by oral gavage started at the age of 12 weeks and continued until 16 weeks of age along with HFD feeding. We found that BDM-X improved the histopathological changes, serum aminotransferases, and blood glucose levels in NASH mice. The hepatic protein expressions of SIRT1 and IL-10 were significantly repressed in NASH mice. BDM-X treatment restored these expressions. BDM-X treatment effectively reduced the progression of NASH by suppressing the protein expression of SREBPlc, p-NF-κB, Ep-CAM, and prothrombin in the NASH liver. In conclusion, our data suggest that BDM-X can protect the liver against inflammation and lipogenesis in NASH-HCC mice.
Comparing the Cosmetic Effects of Liquid-Fermented Culture of Some Medicinal Mushrooms Including Antioxidant, Moisturizing, and Whitening Activities
693-703
10.1615/IntJMedMushrooms.2020035407
Shanshan
Wang
School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, Shandong, P.R. China
Chenbai
Wang
Hongdao People's Hospital of Qingdao, Qingdao 266112, Shandong, P.R. China
Hui
Cao
School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, Shandong, P.R. China
Xiaowei
Cui
School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, Shandong, P.R. China
Hong
Guo
School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, Shandong, P.R. China
Wenxiu
Zheng
School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, Shandong, P.R. China
Xiaofei
Zhong
School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, Shandong, P.R. China
Yongqing
Zhang
School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, Shandong, P.R. China
Chunchao
Han
School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, People's Republic of China; Shandong Provincial Collaborative Innovation Center for Quality Control and Construction of the Whole Industrial Chain of Traditional Chinese Medicine, Jinan, Shandong, 250355, People's Republic of China
cosmetic
Coprinus comatus
Cordyceps militaris
Pleurotus geesteranus
antioxidant
moisturizing
whitening
medicinal mushrooms
There have been few studies on the cosmetic efficacy of Coprinus comatus, Cordyceps militaris, and Pleurotus geesteranus. This article aims to compare the cosmetic effects of the mycelium, fermentation broth, and mycelium and fermentation broth mixture of liquid-fermented C. comatus, C. militaris, and P. geesteranus. Our results verified that these three edible mushrooms all showed excellent antioxidant, moisturizing, and whitening effects. Comparatively, we found that the cosmetic effects of C. comatus were superior to C. militaris and P. geesteranus. Therefore, we speculate that among the three edible mushrooms, C. comatus will be the most suitable to be used as a basis for cosmeceutical formulations to tackle skin aging and hyperpigmentation. This research aims to provide a basis for the study of the cosmetic effects of edible mushrooms.
Effect of Shading and Forest Type on Morphological Characteristics and Bioactive Compounds of Fruiting Bodies of Songshan Lingzhi Ganoderma tsugae (Agaricomycetes)
705-717
10.1615/IntJMedMushrooms.2020035405
Qing-hua
Ma
Experimental Centre of Forestry in North China, Chinese Academy of Forestry, Beijing 102300, P.R. China
Xing-hong
Wang
Experimental Centre of Forestry in North China, Chinese Academy of Forestry, Beijing 102300, P.R. China
Yu-guang
Fan
Changbai Mountain Academy of Sciences, Jilin Provincial Joint Key Laboratory of Changbai Mountain Biocoenosis and Biodiversity, Jilin 130000, P.R. China
Lu-Sen
Bian
Experimental Centre of Forestry in North China, Chinese Academy of Forestry, Beijing 102300, P.R. China
Lin
Chen
Experimental Centre of Forestry in North China, Chinese Academy of Forestry, Beijing 102300, P.R. China
Hui-hui
Liu
Experimental Centre of Forestry in North China, Chinese Academy of Forestry, Beijing 102300, P.R. China
Fei
Ren
Experimental Centre of Forestry in North China, Chinese Academy of Forestry, Beijing 102300, P.R. China
Yong-an
Zhang
Experimental Centre of Forestry in North China, Chinese Academy of Forestry, Beijing 102300, P.R. China
songshan lingzhi
Ganoderma tsugae
cultivation
amino acids
polysaccharides
triterpenes
medicinal mushrooms
Ganoderma tsugae strain MCCCMAS0053 cultivation on short logs results in varied yield and quality under different growth conditions. Thus, growth conditions need optimization to increase yield and quality. An indoor experiment with three shade treatments (A1, two layers of black sun-shade net; A2, one layer of black sun-shade net plus plastic mulch; A3, one layer of black sun-shade net) and a field experiment in two forest types (pine or mixed pine-oak) were conducted. The results showed that shading and forest type significantly affected light intensities and the growth, size, biomass, and bioactive components of G. tsugae fruiting bodies. In the indoor experiment, the mean dry weight of the fruiting body and the diameter of the pileus in A2 increased by 21.51-44.98% and 13.42-22.26%, respectively, compared with those of A1 and A3. Similarly, the accumulation of the bioactive compounds (polysaccharides, total amino acids, and total essential amino acids) in the pileus and stipe were greater in A2 than in A1 and A3. Furthermore, compared with pure pine forest cultivation, fruiting bodies cultivated in mixed pine-oak forest had greater dry weight, pileus diameter, and more bioactive compounds. In addition, no significant difference was found between the A2 and mixed pine-oak treatments, which had a similar light intensity (from 1116 to 2367 lx). Hence, this suggests that the A2 shade treatment or cultivation in mixed pine-oak forest is beneficial for production of G. tsugae fruiting bodies, and light intensity may play a critical role in this process.