RT Journal Article
ID 60d075c07798ee49
A1 Lackmann, Jan-Wilm
A1 Edengeiser, Eugen
A1 Schneider, Simon
A1 Benedikt, Jan
A1 Havenith, Martina
A1 Bandow, Julia E.
T1 Effects of the Effluent of a Microscale Atmospheric Pressure Plasma-jet Operated with He/O2 Gas on Bovine Serum Albumin
JF Plasma Medicine
JO PMED
YR 2013
FD 2014-10-01
VO 3
IS 1-2
SP 115
OP 124
K1 Atmospheric pressure plasma
K1 X-jet
K1 protein modification
K1 BSA
K1 Raman spectroscopy
AB Cold atmospheric pressure plasmas (CAPs) are being investigated for medical applications, and the first clinical studies are promising. However, interactions between plasmas and biological samples are only partly understood on a molecular level. In this study, bovine serum albumin (BSA), a standard model for plasma-mediated etching of biological samples, was used to investigate the effects of different components of an He/O2 plasma effluent on proteins. The X-jet features an optional lateral helium flow that splits the plasma effluent into particles and (V)UV radiation. BSA samples were exposed separately to plasma-emitted particles, UV radiation, or the combination of both. Afterward, plasma-treated samples were investigated using SDS-PAGE and western blot analysis for amino acid strand breaks, but none were detected. Furthermore, treated samples were investigated by Raman spectroscopy to search for chemical modifications. We found that treatment with the X-jet has little effect on BSA. Minor changes in the Raman spectra suggest modifications of tyrosine residues and some degree of oxidation of sulfur-containing amino acids. Our findings suggest that for the X-jet effluent, etching is the main effect of plasma on BSA, making BSA a suitable model with which to study protein etching.
PB Begell House
LK https://www.dl.begellhouse.com/journals/5a5b4a3d419387fb,4eec539e3367d43b,60d075c07798ee49.html