RT Journal Article ID 5cb3eedc0af538ad A1 Klarer, Alden C. A1 McGregor, W. Glenn T1 Replication of Damaged Genomes JF Critical Reviews™ in Eukaryotic Gene Expression JO CRE YR 2011 FD 2011-12-12 VO 21 IS 4 SP 323 OP 336 K1 Y-family DNA polymerases K1 mutagenesis K1 UV radiation K1 DNA damage AB Cellular DNA is continuously assaulted by chemical and physical agents that arise from both endogenous metabolic processes as well as exogenous insults. Commonly encountered environmental agents include polyaromatic hydrocarbons, polycyclic aromatic amines, the ultraviolet component of sunlight, and ionizing radiation, among many others. Although the kinds of damages and the mechanisms involved in their interaction with DNA vary widely, genotoxic agents alter the structure of DNA in ways that may result in permanent alterations in the DNA sequence or in cell death. To avoid these consequences, cells have evolved countermeasures to reduce the biological consequences of DNA damage. These mechanisms are highly conserved and are present in all eukaryotic cells. In general, cellular responses include the detection of damage, signal transduction to halt cell cycle progression, and the recruitment of repair mechanisms that are tailored to the specific kind of damage. If replication-blocking damage remains when cells enter S-phase, then tolerance mechanisms in the form of complex recombination mechanisms or translesion DNA synthesis using accessory DNA polymerases exist. These mechanisms complete the replication of damaged genomes and suppress cytotoxicity, but at the potential cost of mutagenesis and genomic instability. This review focuses on error-prone mechanisms, including a discussion of the Y-family of DNA polymerases, current concepts of DNA polymerase switching mechanisms, and their relevance to cancer and cancer prevention. PB Begell House LK https://www.dl.begellhouse.com/journals/6dbf508d3b17c437,5879f5f63658cafa,5cb3eedc0af538ad.html