RT Journal Article
ID 5988a93154e863c9
A1 Bharati, Sanjay
A1 Rishi, Parveen
A1 Koul, Ashwani
T1 Fourier Transform Infrared Spectroscopic Studies on Modulation of N-Nitrosodiethylamine-Induced Hepatocarcinogenesis by Azadirachta indica
JF Journal of Environmental Pathology, Toxicology and Oncology
JO JEP(T)
YR 2015
FD 2015-06-23
VO 34
IS 3
SP 227
OP 236
K1 chemoprevention
K1 carcinogen metabolism
K1 hepatic tumors
K1 hepatocellular carcinoma
AB Fourier transform infrared spectroscopy was employed in the present study to obtain information about the molecular composition of hepatic tumor versus hepatic tissue. A hepatic cancer model was developed by administering N-nitrosodiethylamine (NDEA) to male Balb/c mice. The results revealed that NDEA-induced hepatic cancer tumor tissue had altered molecular composition compared with normal liver tissue. Compared with the normal
tissue, the saturation level of membrane phospholipids was observed to be decreased in tumors along with an abnormal distribution of protein secondary structures. A significant decrease in glycogen and a significant increase in total nucleic acid content were also observed in tumor cells. The administration of aqueous Azadirachta indica leaf extract (AAILE) prior to NDEA treatment resulted in the normalization of saturation levels in phospholipids and total nucleic acid content and in the distribution of protein secondary structures in tumors. A significant increase in the amount of stored glycogen was observed in AAILE cotreated tumors compared with NDEA-induced tumors, which might indicate that AAILE cotreatment impeded the ability of tumor cells to consume glucose at a faster rate. The normalization of molecular composition upon AAILE cotreatment in hepatic tumors might indicate that AAILE hampered the process of evolution of tumors, which could be responsible for its observed chemopreventive action.
PB Begell House
LK https://www.dl.begellhouse.com/journals/0ff459a57a4c08d0,5e769fe6726f83d4,5988a93154e863c9.html