RT Journal Article ID 4815efdd3cafae8a A1 Elisashvili, Vladimir I. A1 Chichua, David A1 Kachlishvili, Eva A1 Tsiklauri, Nino A1 Khardziani, Tamar T1 Lignocellulolytic Enzyme Activity During Growth and Fruiting of the Edible and Medicinal Mushroom Pleurotus ostreatus (Jacq.:Fr.) Kumm. (Agaricomycetideae) JF International Journal of Medicinal Mushrooms JO IJM YR 2003 FD 2003-06-01 VO 5 IS 2 OP 6 AB Cotton wastes can be converted without substrate pasteurization into value-added products, such as gourmet mushrooms, with biological efficacy up to 90% and lignocellulolytic enzymes. Edible and medicinal mushroom Pleurotus ostreatus carboxymethyl cellulase and laccase activities, extracted from both the surface and inside layers of blocks, increased during primordia and fruiting bodies development and declined rapidly after the harvest. In a study of xylanase production, it has been observed that the activity of the enzyme in inside layers of the substrate gradually increased during the 49 days (second fruiting stage) after inoculation and then gradually declined towards the end of mushroom cultivation. Fluctuations in xylanase activity in the surface layer were observed, so that maximal enzyme activities coincided with the fruiting stage of P. ostreatus development. In contrast to laccase, manganese peroxidase (MnP) activity was high during the colonization stage and declined during the first primordia and fruiting body formation stages. After this, while laccase activity decreased, MnP activity peaked at the mycelia stage in oyster mushroom development. Data on the levels of cellulase, xylanase, laccase, and MnP activity in spent mushroom substrate demonstrate that it can be used as an excellent source of these enzymes. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,3389befb6be7818a,4815efdd3cafae8a.html