RT Journal Article
ID 2070e40d779d0bf7
A1 Li, Zhiguo
A1 Mei, Junjie
A1 Jiang, Liping
A1 Geng, Chengyan
A1 Li, Qiujuan
A1 Yao, Xiaofeng
A1 Cao, Jun
T1 Chaga Medicinal Mushroom, Inonotus obliquus (Agaricomycetes) Polysaccharides Suppress Tacrine-induced Apoptosis by ROS-scavenging and Mitochondrial Pathway in HepG2 Cells
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2019
FD 2019-06-05
VO 21
IS 6
SP 583
OP 593
K1 apoptosis
K1 antioxidant
K1 Inonotus obliquus
K1 medicinal mushrooms
K1 mitochondrial pathway
K1 tacrine
AB Tacrine is the first drug licensed for the treatment of Alzheimer disease. Unfortunately, reversible hepatotoxicity
limits its clinical use. In our previous study, we found that tacrine induced apoptosis in HepG2 cells by reactive oxygen species (ROS) formation and mitochondria dysfunction. Inonotus obliquus is a mushroom traditionally used as a folk medicine in Asia. In this study, the possible protective effect of polysaccharides from I. obliquus was investigated. The results showed that I. obliquus polysaccharides (IOP) reduced tacrine-induced apoptosis in HepG2 cells. Inhibition of tacrine-induced ROS generation, 8-OHdG formation in mitochondrial DNA, and loss of the mitochondrial transmembrane potential by IOP were also observed. Furthermore, IOP decreased the cytochrome c release and activation of caspase-3 induced by tacrine. These data suggest that IOP could inhibit tacrine-induced apoptosis in HepG2 cells. The protection is mediated by an antioxidant protective mechanism. Consumption of IOP may be a plausible way to prevent tacrine-induced hepatotoxicity.
PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,27c0458a59ba057c,2070e40d779d0bf7.html