%0 Journal Article %A Cai, Ming %A Lin, Yang %A Luo, Yin-long %A Liang, Han-hua %A Sun, Peilong %D 2015 %I Begell House %K medicinal mushrooms, Auricularia auricula-judae, crude polysaccharides, antimicrobial, antioxidant %N 6 %P 591-600 %R 10.1615/IntJMedMushrooms.v17.i6.90 %T Extraction, Antimicrobial, and Antioxidant Activities of Crude Polysaccharides from the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Higher Basidiomycetes) %U https://www.dl.begellhouse.com/journals/708ae68d64b17c52,52f67ca6221cc3fa,1ed5215d4bef89c8.html %V 17 %X In this study, crude polysaccharides of culinary-medicinal mushroom Auricularia auricular-judae were extracted by hot water extraction and alcohol precipitation, and their antimicrobial and antioxidant activities were investigated. An optimum extraction condition was obtained at a ratio of liquid to solid 70 mL/g, temperature 90°C, time 4 h and extraction number 4. Accordingly, the best yield of crude polysaccharides was 6.89% with 76.12% in purity. Some bacteria and fungi were used for antimicrobial studies. It was found that crude A. auricula-judae had great antimicrobial activities against Escherichia coli and Staphylococcus aureus, but no activities on the others. The inhibitory diameters of antimicrobial zones for the two were 5.55 ± 0.182 and 9.84 ± 0.076 mm, respectively. Moreover, crude A. auricula-judae had significant antioxidant activities in scavenging free radicals, reducing power assays, and Fe2+ chelating ability assay. Results revealed that crude A. auricula-judae has a great potential as antimicrobial and antioxidant, and it can be a supplementary food for human health. %8 2015-06-30