RT Journal Article ID 0092270b77a07187 A1 Wang, Junzhi A1 Lv, Huifang A1 Chen, Beiyan A1 Huang, Wenfeng A1 Wang, Ailing A1 Liu, Lanqing A1 He, Haibo A1 Chen, Jianfeng A1 Li, Shi A1 Deng, Wei-Qiao T1 Methyl-Hispolon from Phellinus lonicerinus (Agaricomycetes) Affects Estrogen Signals in MCF-7 Breast Cancer Cells and Premature Aging in Rats JF International Journal of Medicinal Mushrooms JO IJM YR 2019 FD 2019-04-11 VO 21 IS 4 SP 381 OP 392 K1 methyl-hispolon K1 estrogen signals K1 phytoestrogen K1 hormone replacement therapy K1 Phellinus lonicerinus K1 medicinal mushrooms AB We studied Phellinus lonicerinus to determine the cytotoxic effect and the dual estrogenic activities of methyl-hispolon and their relation to estrogen signals in vivo and in vitro. The Glide scores of methyl-hispolon-estrogen receptor α (ERα) and methyl-hispolon-ERβ docked complexes were -7.29 kcal/mol and -6.68 kcal/mol in docking simulations. Methyl-hispolon had a significant antiproliferative effect for estrogen-sensitive ER(+) MCF-7 cells in the absence of estrogen, and it exhibited dual estrogen activities. Methyl-hispolon increased the serum E2 in rats with premature ovarian failure and fulfilled the estrogenic function in the uterus and ovary. Methyl-hispolon significantly inhibited the expression of Ras, API, ERα, C-myc, and cyclinDl, as well as their gene transcription in RL95-2 cells. The phosphorylation of ERK1/2 was inhibited by methyl-hispolon. Thus, methyl-hispolon has potential use in treating estrogen deficiency-related diseases, with good antitumor effects and estrogenic activity. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,2b9ba90674ebcc50,0092270b77a07187.html