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International Journal of Medicinal Mushrooms
Facteur d'impact: 1.423 Facteur d'impact sur 5 ans: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN Imprimer: 1521-9437
ISSN En ligne: 1940-4344

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International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.2020034223
pages 389-396

Ergosterol Peroxide from the Egyptian Red Lingzhi or Reishi Mushroom, Ganoderma resinaceum (Agaricomycetes), Showed Preferred Inhibition of MCF-7 over MDA-MB-231 Breast Cancer Cell Lines

Neveen F. El-Sherif
Department of Pharmacognosy, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt
Safwat A. Ahmed
Department of Pharmacognosy, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt
Amany K. Ibrahim
Department of Pharmacognosy, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt
Eman S. Habib
Department of Pharmacognosy, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt
Amira A. El-Fallal
Botany and Microbiology Department, Faculty of Science, Damietta University, New Damietta City, Egypt
Ahmed K. El-Sayed
Botany and Microbiology Department, Faculty of Science, Damietta University, New Damietta City, Egypt
Amir E. Wahba
Chemistry Department, Faculty of Science, Damietta University, New Damietta City, Egypt; Department of Neurosurgery, Robert Wood Johnson Medical School, Rutgers University, Piscataway, NJ 08854, USA


Ergosterol peroxide and ganoderic acid AMI were isolated for the first time from the mycelium of the Egyptian Ganoderma resinaceum mushroom. The structure of these two metabolites was established by detailed analysis of 1D and 2D NMR. The isolated compounds were tested for their antitumor in vitro activities in MCF-7 and MDA-MB-231 breast cancer cell lines. Ergosterol peroxide showed preferred inhibition of MCF-7 (ER +ve) cell lines relative to MDA-MB-231 (ER -ve) cell lines with an IC50 of 1.18 μM and 12.82 μM respectively. Our data suggest that ergosterol peroxide targets estrogen receptors.


  1. Chen Y-K, Kuo Y-H, Chiang B-H, Lo J-M, Sheen L-Y. Cytotoxic activities of 9,11-dehydroergosterol peroxide and ergosterol peroxide from the fermentation mycelia of ganoderma lucidum cultivated in the medium containing leguminous plants on Hep 3B cells. J Agric Food Chem. 2009;57(13):5713-9.

  2. Kobori M, Yoshida M, Ohnishi-Kameyama M, Shinmoto H. Ergosterol peroxide from an edible mushroom suppresses inflammatory responses in RAW264.7 macrophages and growth of HT29 colon adenocarcinoma cells. Br J Pharmacol. 2007;150(2):209-19.

  3. Kovacs B, Beni Z, Dekany M, Bozsity N, Zupko I, Hohmann J, Vanyolos A. Isolation and structure determination of anti-proliferative secondary metabolites from the potato earthball mushroom, Scleroderma bovista (Agaricomycetes). Int J Med Mushrooms. 2018;20(5):411-18.

  4. Mendoza G, Suarez-Medellin J, Espinoza C, Ramos-Ligonio A, Fernandez JJ, Norte M, Trigos A. Isolation and characterization of bioactive metabolites from fruiting bodies and mycelial culture of Ganoderma oerstedii (higher Basidiomycetes) from Mexico. Int J Med Mushrooms. 2015;17(6):501-9.

  5. Ma L, Chen H, Dong P, Lu X. Anti-inflammatory and anticancer activities of extracts and compounds from the mushroom Inonotus obliquus. Food Chem. 2013;139(1):503-8.

  6. Dembitsky VM. Bioactive peroxides as potential therapeutic agents. Eur J Med Chem. 2008;43(2):223-51.

  7. Merdivan S, Lindequist U. Ergosterol peroxide: A mushroom-derived compound with promising biological activities: A review. Int J Med Mushrooms. 2017;19(2):93-105.

  8. Tan W, Pan M, Liu H, Tian H, Ye Q, Liu H. Ergosterol peroxide inhibits ovarian cancer cell growth through multiple pathways. OncoTargets Ther. 2017;10:3467-74.

  9. Li X, Wu Q, Bu M, Hu L, Du WW, Jiao C, Pan H, Sdiri M, Wu N, Xie Y, Yang BB. Ergosterol peroxide activates Foxo3-mediated cell death signaling by inhibiting AKT and c-Myc in human hepatocellular carcinoma cells. Oncotarget. 2016;7(23):33948-59.

  10. Han J, Sohn EJ, Kim B, Kim S, Won G, Yoon S, Lee J, Kim MJ, Lee H, Chung K, Kim SH. Upregulation of death receptor 5 and activation of caspase 8/3 play a critical role in ergosterol peroxide induced apoptosis in DU 145 prostate cancer cells. Cancer Cell Int. 2014;14(1):117.

  11. Zheng L, Si J, Wong Y-S. Ergosterol peroxide and 9,11-dehydroergosterol peroxide from ling zhi or reishi medicinal mushroom, Ganoderma lucidum (W. Curt.: Fr.) P. Karst. (Aphyllophoromycetideae) mycelia inhibit the growth of human breast adenocarcinoma MCF-7 Cells. Int J Med Mushrooms. 2009;11(3):249-57.

  12. Rhee Y-H, Jeong S-J, Lee H-J, Lee H-J, Koh W, Jung JH, Kim S, Sun-Hoon K. Inhibition of STAT3 signaling and induction of SHP1 mediate antiangiogenic and antitumor activities of ergosterol peroxide in U266 multiple myeloma cells. BMC Cancer. 2012;12:28.

  13. Wu Q-P, Xie Y-Z, Deng Z, Li X-M, Yang W, Jiao C-W, Fang L, Li S, Pan, H, Yee AJ, Lee DY, Li C, Zhang Z, Guo J, Yang BB. Ergosterol peroxide isolated from Ganoderma lucidum abolishes microRNA miR-378-mediated tumor cells on chemore-sistance. PLoS One. 2012;7(8):e44579.

  14. Sedky NK, Gammal ZHE, Wahba AE, Mosad E, Waly ZY, El-Fallal AA, Arafa RK, El-Badri N. The molecular basis of cytotoxicity of a-spinasterol from Ganoderma resinaceum: Induction of apoptosis and overexpression of p53 in breast and ovarian cancer cell lines. J Cell Biochem. 2018;119(5):3892-902.

  15. Paterson RRM. Ganoderma- a therapeutic fungal biofactory. Phytochemistry. 2006;67(18):1985-2001.

  16. Rahman MA, Abdullah N, Aminudin N. Evaluation of the antioxidative and hypo-cholesterolemic effects of Lingzhi or Reishi medicinal mushroom, Ganoderma lucidum (Agaricomycetes), in ameliorating cardiovascular disease. Int J Med Mushrooms. 2018;20(10):961-69.

  17. Rubel R, Santa HSD, dos Santos LF, Fernandes LC, Figueiredo BC, Soccol CR. Immunomodulatory and antitumoral properties of Ganoderma lucidum and Agaricus brasiliensis (Agaricomycetes) medicinal mushrooms. Int J Med Mushrooms. 2018;20(4):393-403.

  18. Nowak R, Drozd M, Mendyk E, Lemieszek M, Krakowiak O, Kisiel W, Rzeski W, Szewczyk K. A new method for the isolation of ergosterol and peroxyergosterol as active compounds of hygrophoropsis aurantiaca and in vitro antiproliferative activity of isolated ergosterol peroxide. Molecules. 2016;21(7):946.

  19. Chun-Nan L, Shih-Hsieh K, Shen-Jeu W. Steroids of formosan Ganoderma amboinense. Phytochemistry. 1993;32(6):1549-51.

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