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International Journal of Medicinal Mushrooms
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ISSN Imprimer: 1521-9437
ISSN En ligne: 1940-4344

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International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v15.i5.80
pages 505-515

Mycelial Biomass and Biochemical Properties of Proteases Produced by Lentinus citrinus DPUA 1535 (Higher Basidiomycetes) in Submerged Cultivation

Larissa de Souza Kirsch
Culture Collection DPUA/UFAM, Federal University of Amazon, Manaus, AM, Brazil
Valeria de Carvalho Santos Ebinuma
Department of Bioprocess and Biotechnology, School of Pharmaceutical Sciences, UNESP-Univ. Estadual Paulista, Araraquara, Brazil
Maria Francisca Simas Teixeira
Culture Collection DPUA/UFAM, Federal University of Amazon, Manaus, AM, Brazil

RÉSUMÉ

The cultivation of Lentinus citrinus for mycelial biomass and protease production under different carbon and nitrogen sources was studied in submerged cultivation. The nutritional source concentration for protease production was evaluated using a full factorial design. For mycelial biomass maltose (4.94 mg/mL) and beef extract (5.45 mg/mL), carbon and nitrogen sources presented the best results, respectively. The maximum protease activity was 73.33 U/mL with fructose (30.0 g/L) and beef extract (10.0 g/L). Proteases showed maximum activity at 40°C and pH 7.0, which exhibited high stability at experimental conditions. The final part of this work was devoted to estimating the main thermodynamic parameters of the irreversible enzyme inactivation (ΔH* = 17.86 kJ/mol, ΔG* =102.09 kJ/mol, ΔS* = −260.76 J/mol×K) through residual activity tests carried out at 25−70°C, by making use of Arrhenius and Eyring plots.