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International Journal of Medicinal Mushrooms
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ISSN Imprimer: 1521-9437
ISSN En ligne: 1940-4344

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International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v8.i3.50
pages 231-244

Comparison of the Immunomodulating Activities of 1,3-β-glucan Fractions from the Culinary-Medicinal Mushroom Sparassis crispa Wulf.:Fr. (Aphyllophoromycetideae)

Toshie Harada
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan
Hiromi Kawaminami
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy & Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan
Noriko N. Miura
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Tokyo, Japan
Yoshiyuki Adachi
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, Japan
Mitsuhiro Nakajima
Minahealth Co. Ltd., Saitama, Japan
Toshiro Yadomae
Laboratory of Immunopharmacology of Microbial Products, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan
Naohito Ohno
Laboratory for Immunopharmacology of Microbial Products, Tokyo University of Pharmacy and Life Science, Tokyo, Japan

RÉSUMÉ

Sparassis crispa is a culinary-medicinal mushroom recently cultivated in Japan, China, Germany, and the U.S. In a previous study, we purified a 6-branched 1,3-β-D-glucan preparation, designated as SCG, and investigated its biological activities. In the present study, we prepared polysac-charide fractions extracted from S. crispa by hot water (SCHWE), cold NaOH (SCG), and hot NaOH (SCHA), and investigated their properties and immunomodulating activities. These polysaccharide fractions showed strong limulus factor G activity due to the presence of β-1,3-D-glucans. They also showed a strong antitumor activity against the solid form of Sarcoma 180 in ICR mice, and hemato-poietic response in cyclophosphamide-induced leukopenic mice. They induced the splenocytes from DBA/2 mice to produce GM-CSF, TNF-α, and IFN-γ in vitro. By treatment with sodium hydroxide of SCHWE, the hematopietic response was enhanced but cytokine induction was decreased. Taken together, these results strongly suggested that the preparation method was related to the conformation of the β-glucan fraction, and the immunomodulating activities in vivo and in vitro were significantly modulated by the preparation methods.


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