Publication de 12 numéros par an
ISSN Imprimer: 1521-9437
ISSN En ligne: 1940-4344
Indexed in
Identification and Evaluation of Reference Genes for qRT-PCR Normalization in Sparassis latifolia (Agaricomycetes)
RÉSUMÉ
Real-time quantitative polymerase chain reaction (qRT-PCR) has emerged as a powerful and popular tool for quantitating differences in transcriptional gene expression levels between samples. Validation of the stability of reference genes is a fundamental step before initiating qRT-PCR assays. Sparassis latifolia is an edible and medicinal fungus containing a remarkably high concentration of β-glucan, which has many biological and pharmacologic activities. S. latifolia may be a model species for studying fungal photobiology because its fruiting body formation requires more light than other fungi. However, suitable reference genes for qRT-PCR have not yet been determined. In the present study, 10 candidate reference genes in S. latifolia were evaluated and validated under different developmental stages and light conditions. To evaluate the suitability of candidate reference genes, three popular software programs (geNorm, NormFinder, and BestKeeper), along with the delta Ct method, were used to analyze these genes; the final ranking was determined using RefFinder. According to our results, Actin and GAPDH were expressed at the most stable levels under different developmental stages and light conditions.
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Yang Chi, Ma Lu, Xiao Donglai, Ying Zhenghe, Jiang Xiaoling, Lin Yanquan, Integration of ATAC-Seq and RNA-Seq Identifies Key Genes in Light-Induced Primordia Formation of Sparassis latifolia, International Journal of Molecular Sciences, 21, 1, 2019. Crossref
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Wang Gangzheng, Cheng Huijiao, Li Min, Zhang Chenghua, Deng Wangqiu, Li Taihui, Selection and validation of reliable reference genes for Tolypocladium guangdongense gene expression analysis under differentially developmental stages and temperature stresses, Gene, 734, 2020. Crossref
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Lu Yuan-Ping, Liao Jian-Hua, Guo Zhong-Jie, Zheng Hui-Qing, Lu Ling-Fang, Cai Zhi-Xin, Zeng Zhi-Heng, Ying Zheng-He, Chen Mei-Yuan, Banche Giuliana, Selection and Evaluation of Potential Reference Genes for Quantitative Real-Time PCR in Agaricus blazei Based on Transcriptome Sequencing Data, BioMed Research International, 2021, 2021. Crossref
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Yang Chi, Ma Lu, Xiao Donglai, Liu Xiaoyu, Jiang Xiaoling, Lin Yanquan, Comparative transcriptomics reveals unique pine wood decay strategies in the Sparassis latifolia, Scientific Reports, 12, 1, 2022. Crossref