Publication de 12 numéros par an
ISSN Imprimer: 1521-9437
ISSN En ligne: 1940-4344
Indexed in
Cloning and Characterization of Laccase DNA from the Royal Sun Medicinal Mushroom, Agaricus brasiliensis (Higher Basidiomycetes)
RÉSUMÉ
Laccase isozymes have been identified in several fungi. We report the cloning of 4 laccase genes from the medicinal mushroom Agaricus brasiliensis. The lac1 gene contained a 1560-base pair (bp) open reading frame (ORF) encoding 520 amino acids that was interrupted with 14 introns in genomic DNA. The deduced amino acid sequence indicated a multicopper oxidase signature 1 and 2 multicopper oxidase signature 2. The lac2 gene contained a 1566-bp ORF encoding 522 amino acids that was interrupted with 13 introns in genomic DNA. A number of different nucleotides were observed in whole regions containing the substitution of amino acid residues (lac2a and lac2b). The partial DNA fragments of lac3 and lac4 genes were subcloned using the semi-random two-step polymerase chain reaction method. The lac3 and lac4 genes contained coding sequences with a 1575-bp ORF encoding 525 amino acids and a 1584-bp ORF encoding 528 amino acids, respectively. However, the whole complementary DNA fragment of both laccases could not be amplified with polymerase chain reaction against the complementary DNA library; therefore, introns were deduced based on the GT-AG rule and multiple alignment of laccases from other fungi, which showed high identity. All laccases from A. brasiliensis conserved the fungal laccase signature sequence and suggest 2 subfamilies according to the location of introns and phylogenetic analysis. The genes lac2 and lac4 had a high degree of identity, and the lac2a gene was located upstream of the lac4 gene.
-
Tajima Katsuya, Akanuma Satoshi, Matsumoto-Akanuma Akiko, Yamanaka Daisuke, Ishibashi Ken-ichi, Adachi Yoshiyuki, Ohno Naohito, Activation of macrophages by a laccase-polymerized polyphenol is dependent on phosphorylation of Rac1, Biochemical and Biophysical Research Communications, 495, 3, 2018. Crossref
-
Hamuro Yuhi, Tajima Katsuya, Matsumoto-Akanuma Akiko, Sakamoto Sayaka, Furukawa Ryutaro, Yamagishi Akihiko, Ohno Naohito, Akanuma Satoshi, Characterization of a thermostable mutant of Agaricus brasiliensis laccase created by phylogeny-based design, Journal of Bioscience and Bioengineering, 124, 6, 2017. Crossref
-
Kim Seonghun, Mushroom Ligninolytic Enzymes―Features and Application of Potential Enzymes for Conversion of Lignin into Bio-Based Chemicals and Materials, Applied Sciences, 11, 13, 2021. Crossref