Publication de 12 numéros par an
ISSN Imprimer: 1521-9437
ISSN En ligne: 1940-4344
Indexed in
Isolation and Characterization of a Ubiquitin-Like Ribonuclease from the Cultured Deep Root Mushroom, Oudemansiella radicata (Higher Basidiomycetes)
RÉSUMÉ
The isolation of a novel 13.5-kDa ribonuclease, displaying a ubiquitin-like inner peptide sequence, from dried fruiting bodies of the cultured mushroom Oudemansiella radicata (Relhan: Fr.) Singer (=Xerula radicata) is reported. The purification protocol deployed encompassed sequentially, cation/anion exchange chromatography on CM-cellulose, DEAE-cellulose and SP-Sepharose, and FPLC-gel filtration on a Superdex 75 column. The purified enzyme manifested optimum activity at 70°C and pH 4.6, respectively. The activity of the RNase was inhibited by the majority of metal ions tested, especially Al3+, Hg2+, and Cd2+ ions, but was promoted by K+ ions. It exhibited the highest ribonucleolytic activity toward poly (C), lower activity toward poly (G), and negligible activity toward poly (U) and poly (A). Compared with mushroom ubiquitin-like RNases reported earlier, O. radicata RNase possesses a larger molecular mass, distinctive chromatographic behavior on DEAE-cellulose, a lower optimum pH, and a unique polyhomoribonucleotide specificity.
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Liu Qin, Zhu Mengjuan, Geng Xueran, Wang Hexiang, Ng Tzi, Characterization of Polysaccharides with Antioxidant and Hepatoprotective Activities from the Edible Mushroom Oudemansiella radicata, Molecules, 22, 2, 2017. Crossref
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Rezvani Vala, Pourianfar Hamid R., Mohammadnejad Safoora, Madjid Ansari Alireza, Farahmand Leila, Anticancer potentiality and mode of action of low-carbohydrate proteins and peptides from mushrooms, Applied Microbiology and Biotechnology, 104, 16, 2020. Crossref
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Geng Xueran, Lei Jiayu, Bau Tergun, Guo Dongdong, Chang Mingchang, Feng Cuiping, Xu Lijing, Cheng Yanfen, Zuo Ningke, Meng Junlong, Purification, Characterization, and Immobilization of a Novel Protease-Resistant α-Galactosidase from Oudemansiella radicata and Its Application in Degradation of Raffinose Family Oligosaccharides from Soymilk, Foods, 11, 19, 2022. Crossref