Abonnement à la biblothèque: Guest
Portail numérique Bibliothèque numérique eBooks Revues Références et comptes rendus Collections
International Journal of Medicinal Mushrooms
Facteur d'impact: 1.423 Facteur d'impact sur 5 ans: 1.525 SJR: 0.433 SNIP: 0.661 CiteScore™: 1.38

ISSN Imprimer: 1521-9437
ISSN En ligne: 1940-4344

Volumes:
Volume 21, 2019 Volume 20, 2018 Volume 19, 2017 Volume 18, 2016 Volume 17, 2015 Volume 16, 2014 Volume 15, 2013 Volume 14, 2012 Volume 13, 2011 Volume 12, 2010 Volume 11, 2009 Volume 10, 2008 Volume 9, 2007 Volume 8, 2006 Volume 7, 2005 Volume 6, 2004 Volume 5, 2003 Volume 4, 2002 Volume 3, 2001 Volume 2, 2000 Volume 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.2017024355
pages 849-859

Functional Properties of Partially Characterized Polysaccharide from the Medicinal Mushroom Ganoderma neo-japonicum (Agaricomycetes)

Sarasvathy Subramaniam
Mushroom Research Centre, University of Malaya, Kuala Lumpur, Malaysia; Department of Biomedical Science, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia
Jegadeesh Raman
Mushroom Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumsung, Republic of Korea
Vikineswary Sabaratnam
Mushroom Research Centre, University of Malaya, Kuala Lumpur, Malaysia; Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia
Chua Kek Heng
Mushroom Research Centre, University of Malaya, Kuala Lumpur, Malaysia; Department of Biomedical Science, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia
Umah Rani Kuppusamy
Mushroom Research Centre, University of Malaya, Kuala Lumpur, Malaysia; Department of Biomedical Science, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia

RÉSUMÉ

This study was conducted to evaluate the mycochemical composition and antiglycemic and antioxidant activities of Ganoderma neo-japonicum hot aqueous extracts, prepared at different boiling durations, and polysaccharides isolated from them. Ground basidiocarps of G. neo-japonicum were double-boiled at 100°C for 0.5, 3, or 4 hours, and the antiglycemic activity was assessed by α-amylase and α-glucosidase enzyme inhibition assays. The antioxidant capacity of the crude hot aqueous extracts (AE-1, AE-2, AE-3) was assessed by DPPH and ABTS radical scavenging and ferric-reducing antioxidant power assays. The total phenolics, protein, and sugar in the crude extracts were also determined. The hot aqueous extract (AE-3) containing a significant amount of total sugar and having enhanced antiglycemic and antioxidant activities was selected for polysaccharide isolation. The isolated crude polysaccharide was separated and purified using diethylaminoethyl-cellulose-52 and Sepharose 6B column chromatography. Fourier transform infrared spectroscopy studies of the purified polysaccharide fraction (PF) showed the presence of typical bands corresponding to polysaccharides. The estimated β-glucan concentration in the PF was 39.26%. In general, the PF exhibited significantly lower antioxidant activity than AE-3. Nevertheless, its potency in inhibiting carbohydratehydrolyzing enzymes may have potential in the management of diabetes mellitus.


Articles with similar content:

Purification, Characterization, Antioxidation, and Antiaging Properties of Exopolysaccharides and Endopolysaccharides of the Royal Sun Medicinal Mushroom, Agaricus brasiliensis (Agaricomycetes)
International Journal of Medicinal Mushrooms, Vol.18, 2016, issue 12
Huijuan Jing, Zhenzhen Ren, Shangshang Li, Zheng Gao, Jianjun Zhang, Le Jia, Shanglong Gao
Antioxidant and Antiproliferative Potential of Fruiting Bodies of the Wild-Growing King Bolete Mushroom, Boletus edulis (Agaricomycetes), from Western Serbia
International Journal of Medicinal Mushrooms, Vol.19, 2017, issue 1
Nebojsa llic, Sonja Kaisarevic, Aleksandra Novakovic, Maja Karaman, Tanja Radusin
Antioxidant, Anticancer, Antimicrobial, and Antibiofilm Properties of the Culinary-Medicinal Fairy Ring Mushroom, Marasmius oreades (Agaricomycetes)
International Journal of Medicinal Mushrooms, Vol.21, 2019, issue 6
Nergiz Demirtas, Naznoosh Shomali, Okan Onar, Ozlem Yildirim, Basar Karaca, Ilgaz Akata, Arzu Coleri Cihan
Determination of the Antioxidant Activity and Polyphenol Contents of Wild Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (W.Curt. Fr.) P. Karst. (Higher Basidiomycetes) from Central Himalayan Hills of India
International Journal of Medicinal Mushrooms, Vol.13, 2011, issue 6
Prem Singh Negi, Zakwan Ahmed, Mohammed Mohsin
Purification, Composition Analysis, and Antioxidant Activity of Exopolysaccharides from Mycelial Culture of Paecilomyces cicadae (Miq.) Samson (Ascomycetes)
International Journal of Medicinal Mushrooms, Vol.12, 2010, issue 1
Xueqian Wu, Junwen Cheng, Xinyan Lu, Haibo Li, Liang He