Abonnement à la biblothèque: Guest
Portail numérique Bibliothèque numérique eBooks Revues Références et comptes rendus Collections
International Journal of Medicinal Mushrooms
Facteur d'impact: 1.423 Facteur d'impact sur 5 ans: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN Imprimer: 1521-9437
ISSN En ligne: 1940-4344

Volumes:
Volume 22, 2020 Volume 21, 2019 Volume 20, 2018 Volume 19, 2017 Volume 18, 2016 Volume 17, 2015 Volume 16, 2014 Volume 15, 2013 Volume 14, 2012 Volume 13, 2011 Volume 12, 2010 Volume 11, 2009 Volume 10, 2008 Volume 9, 2007 Volume 8, 2006 Volume 7, 2005 Volume 6, 2004 Volume 5, 2003 Volume 4, 2002 Volume 3, 2001 Volume 2, 2000 Volume 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.2018028430
pages 1163-1172

Expression and Antitumor Function of Latcripin-4 RCC1 and ANK Domain Protein on HepG2 from the Shiitake Medicinal Mushroom, Lentinus edodes C91–3 (Agaricomycetes), Transcriptome

Xuefang Guo
Department of Medical Microbiology, Dalian Medical University, Lvshunkou District, Dalian, China
Xiaoli Wang
Department of Medical Microbiology, Dalian Medical University, Lvshunkou District, Dalian, China
Min Liu
Department of Medical Microbiology, Dalian Medical University, Lvshunkou District, Dalian, China
Lei Chen
Department of Medical Microbiology, Dalian Medical University, Lvshunkou District, Dalian, China
Warren Chanda
Department of Medical Microbiology, Dalian Medical University, Lvshunkou District, Dalian, China
Arshad Ahmed Pahiar
Department of Medical Microbiology, Dalian Medical University, Lvshunkou District, Dalian, China
Xingyun Li
Department of Medical Microbiology, Dalian Medical University, Lvshunkou District, Dalian, China
Wei Zhang
Department of Medical Microbiology, Dalian Medical University, Lvshunkou District, Dalian, China
Anhong Ning
Department of Medical Microbiology, Dalian Medical University, Lvshunkou District, Dalian, China
Min Huang
Department of Medical Microbiology, Dalian Medical University, Lvshunkou District, Dalian, China
Jing Cao
Department of Medical Microbiology, Dalian Medical University, Lvshunkou District, Dalian, China
Mintao Zhong
Department of Medical Microbiology, Dalian Medical University, Lvshunkou District, Dalian, China

RÉSUMÉ

The recombinant protein of Latcripin-4 regulator of chromosome condensation 1 (RCC1) and ankyrin (ANK) domains were expressed and the antitumor activity of Latcripin-4 on HepG2 cells was studied. First, the Latcripin-4 transcript was selected from the medicinal mushroom Lentinus edodes C91–3 transcriptome by bioinformatics. Then the full-length gene of Latcripin-4 was isolated with 3'-full rapid amplification of cDNA ends (RACE) and 5'-full RACE methods according to the transcriptome. The RCC1 and ANK domains from the full-length gene were selected and inserted into the expression vector pET-32a (+) and expressed in Escherichia coli Rosetta-gami (DE3). Western blotting indicated that the protein was expressed successfully. The biological function of Latcripin-4 RCC1 and ANK domain protein on HepG2 cells was studied with the CCK-8 assay. All results demonstrated that Latcripin-4 RCC1 and ANK domain protein can inhibit the growth of human HepG2 liver cancer cells, which brings new insights to identifying antitumor proteins from medicinal food for cancer therapy.


Articles with similar content:

Locostatin Disrupts Association of Raf Kinase Inhibitor Protein With Binding Proteins by Modifying a Conserved Histidine Residue in the Ligand-Binding Pocket
Forum on Immunopathological Diseases and Therapeutics, Vol.2, 2011, issue 1
Gabriel Fenteany, Christian E. Argueta, Jose A. Gascon, Lochana C. Menikarachchi, Anwar B. Beshir
Posttranslational Regulation of p53 Tumor Suppressor Protein Function
Critical Reviews™ in Oncogenesis, Vol.5, 1994, issue 1
Steve A. Maxwell, Jack A. Roth
Phosphotyrosine Recognition by the Raf Kinase Inhibitor Protein
Forum on Immunopathological Diseases and Therapeutics, Vol.2, 2011, issue 1
Philip C. Simister, Nicholas M. Burton, R. Leo Brady
The Birch Bracket Medicinal Mushroom, Fomitopsis betulina (Agaricomycetes) - Bioactive Source for Beta-Glucan Fraction with Tumor Cell Migration Blocking Ability
International Journal of Medicinal Mushrooms, Vol.22, 2020, issue 1
Miriam Sari, Nicole Teusch, Carolin Roth, Katharina Toepler, Reinhard Hambitzer
The Cbl Family of Signal Transduction Molecules
Critical Reviews™ in Oncogenesis, Vol.8, 1997, issue 4
Linda Smit , Jannie Borst