Publication de 12 numéros par an
ISSN Imprimer: 1521-9437
ISSN En ligne: 1940-4344
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Thirteen-Week Oral Toxicity Evaluation of Erinacine AEnriched Lion's Mane Medicinal Mushroom, Hericium erinaceus (Agaricomycetes), Mycelia in Sprague-Dawley Rats
RÉSUMÉ
Recently, erinacine A-enriched Hericium erinaceus (EAHE) mycelia have demonstrated therapeutic efficacy in animal models of neurodegenerative disease, including Alzheimer and Parkinson disease. Despite promising results from animal models, there have been no reports on its toxicity after long-term consumption. Hence, the present study was designed to evaluate the safety of EAHE mycelia through a 13-week subchronic rodent feeding study. Following 13 weeks of EAHE mycelia feeding at dosages of 0, 875, 1750, and 2625 mg/kg body weight in both male and female Sprague-Dawley rats, findings revealed neither any mortalities nor noticeable toxicological effects in all the rats during the investigation period. Physiological parameters including body weight and feed consumption patterns were unaffected by EAHE mycelia administration. The hematological and biochemical parameters as well as histopathological studies revealed no significant differences between the treatment and control groups. Conclusively, the obtained results suggested that EAHE mycelia could be relatively unharmful when used over an extended period, supporting its safe use in food preparation.
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Mizuno T. , Bioactive substances in Hericium erinaceus (Bull.: Fr.) Pers. (Yamabushitake), and its medicinal utilization. Int J Med Mushrooms. 1999;1(2):105–19.
-
Friedman M. , Chemistry, nutrition, and health-promoting properties of Hericium erinaceus (lion’s mane) mushroom fruiting bodies and mycelia and their bioactive compounds. J Agric Food Chem. 2015;63(32):7108–23.
-
Shimbo M, Kawagishi H, Yokogoshi H. , Erinacine A increases catecholamine and nerve growth factor content in the central nervous system of rats. Nutr Res. 2005;25(6):617–23.
-
Kawagish H, Zhuang C. , Compounds for dementia from Hericium erinaceum. Drugs Future. 2008;33(2):149–55.
-
Obara Y, Nakahata N. , The signaling pathway of neurotrophic factor biosynthesis. Drug News Perspect. 2002;15(5):290–8.
-
Mori K, Inatomi S, Ouchi K, Azumi Y, Tuchida T. , Improving effects of the mushroom Yamabushitake (Hericium erinaceus) on mild cognitive impairment: a double-blind placebo-controlled clinical trial. Phytother Res. 2009;23(3):367–72.
-
Ma BJ, Shen JW, Yu HY, Ruan Y, Wu TT, Zhao X. , Hericenones and erinacines: stimulators of nerve growth factor (NGF) biosynthesis in Hericium erinaceus. Mycology. 2010;1(2):92–8.
-
Mori K, Obara Y, Hirota M, Azumi Y, Kinugasa S, Inatomi S, Nakahata N. , Nerve growth factor-inducing activity of Hericium erinaceus in 1321N1 human astrocytoma cells. Biol Pharm Bull. 2008;31(9):1727–32.
-
Kawagishi H, Shimada A, Shirai R, Okamoto K, Ojima F, Sakamoto H, Ishiguro Y, Furukawa S. , Erinacines a, b and c, strong stimulators of nerve growth factor (NGF)-synthesis, from the mycelia of Hericium erinaceum. Tetrahedr Lett. 1994;35(10):1569–72.
-
Yamada K, Nitta A, Hasegawa T, Fuji K, Hiramatsu M, Kameyama T, Furukawa Y, Hayashi K, Nabeshima T. , Orally active NGF synthesis stimulators: potential therapeutic agents in Alzheimer’s disease. Behav Brain Res. 1997;83(1-2):117–22.
-
Lee KF, Chen JH, Teng CC, Shen CH, Hsieh MC, Lu CC, Lee KC, Lee LY, Chen WP, Chen CC, Huang WS, Kuo HC, Protective effects of Hericium erinaceus mycelium and its isolated erinacine A against ischemia-injury-induced neuronal cell death via the inhibition of iNOS/p38 MAPK and nitrotyrosine. Int J Mol Sci. 2014;15(9):15073–89.
-
Kuo HC, Lu CC, Shen CH, Tung SY, Hsieh MC, Lee KC, Lee LY, Chen CC, Teng CC, Huang WS, Chen TC, Lee KF, Hericium erinaceus mycelium and its isolated erinacine A protection from MPTP-induced neurotoxicity through the ER stress, triggering an apoptosis cascade. J Transl Med. 2016;14:78.
-
Tzeng TT, Chen CC, Lee LY, Chen WP, Lu CK, Shen CC, Huang F. CY, Chen CC, Shiao YJ. , Erinacine A-enriched Hericium erinaceus mycelium ameliorates Alzheimer’s disease-related pathologies in APPswe/PS1dE9 transgenic mice. J Biomed Sci. 2016;23(1):49.
-
Li IC, Chen YL, Lee LY, Chen WP, Tsai YT, Chen CC, Chen CS. , Evaluation of the toxicological safety of erinacine A-enriched Hericium erinaceus in a 28-day oral feeding study in Sprague-Dawley rats. Food Chem Toxicol. 2014;70:61–7.
-
Li IC, Chen YL, Chen WP, Lee LY, Tsai YT, Chen CC, Chen CS. , Genotoxicity profile of erinacine A-enriched Hericium erinaceus mycelium. Toxicol Rep. 2014;1:1195–201.
-
National Research Council. Guide for the care and use of laboratory animals. 8th ed. Washington (DC): National Academies Press; 2011.
-
Organization for Economic Co-operation and Development. Test No. 408: Repeated Dose 90-Day Oral Toxicity Study in Rodents. 1998. Available from: http://dx.doi.org/10.1787/9789264070707-en.
-
FDA. Part 58—Good laboratory practice for nonclinical laboratory studies. 2011. Available from: https://www.accessdata. fda.gov/scripts/cdrh/cfdocs/cfcfr/CFRSearch.cfm?CFRPart=58&showFR=1&subpartNode=21:1.0.1.1.23.7.
-
Piao Y, Liu Y, Xie X. , Change trends of organ weight background data in Sprague Dawley rats at different ages. J Toxicol Pathol. 2013;26(1):29–34.
-
Cummings JL, Morstorf T, Zhong K. , Alzheimer’s disease drug-development pipeline: few candidates, frequent failures. Alzheimers Res Ther. 2014;6(4):37.
-
Lakshmanan H, Raman J, David P, Wong KH, Naidu M, Sabaratnam V. , Haematological, biochemical and histopathological aspects of Hericium erinaceus ingestion in a rodent model: A sub-chronic toxicological assessment. J Ethnopharmacol. 2016;194:1051–9.
-
Martel J, Ojcius DM, Chang CJ, Lin CS, Lu CC, Ko YF, Tseng SF, Lai HC, Young JD. , Anti-obesogenic and antidiabetic effects of plants and mushrooms. Nat Rev Endocrinol. 2017;13(3):149–60.
-
Mukinda JT, Eagles PFK. , Acute and sub-chronic oral toxicity profiles of the aqueous extract of Polygala fruticosa in female mice and rats. J Ethnopharmacol. 2010;128(1):236–40.
-
Levine B. , Animal clinical pathology. In: Derelanko MJ, editor. Handbook of toxicology. 2nd ed. CRC Press (FL): Informa Healthcare; 2001.
-
Giknis M, Clifford C. , Clinical laboratory parameters for Crl: CD(SD) rats. Wilmington (MA): Charles River Laboratories; 2006.
-
Lum G. , Significance of low serum alkaline phosphatase activity in a predominantly adult male population. Clin Chem. 1995;41(4):515–8.
-
Li I-Chen, Chang Han-Hsin, Lin Chuan-Han, Chen Wan-Ping, Lu Tsung-Han, Lee Li-Ya, Chen Yu-Wen, Chen Yen-Po, Chen Chin-Chu, Lin David Pei-Cheng, Prevention of Early Alzheimer’s Disease by Erinacine A-Enriched Hericium erinaceus Mycelia Pilot Double-Blind Placebo-Controlled Study, Frontiers in Aging Neuroscience, 12, 2020. Crossref
-
Limanaqi Fiona, Biagioni Francesca, Busceti Carla Letizia, Polzella Maico, Fabrizi Cinzia, Fornai Francesco, Potential Antidepressant Effects of Scutellaria baicalensis, Hericium erinaceus and Rhodiola rosea, Antioxidants, 9, 3, 2020. Crossref
-
Rai Sachchida Nand, Mishra Divya, Singh Payal, Vamanu Emanuel, Singh M.P., Therapeutic applications of mushrooms and their biomolecules along with a glimpse of in silico approach in neurodegenerative diseases, Biomedicine & Pharmacotherapy, 137, 2021. Crossref
-
Khieokhajonkhet Anurak, Aeksiri Niran, Ratanasut Kumrop, Kannika Korntip, Suwannalers Piluntasoot, Tatsapong Pattaraporn, Inyawilert Wilasinee, Kaneko Gen, Effects of dietary Hericium erinaceus powder on growth, hematology, disease resistance, and expression of genes related immune response against thermal challenge of Nile tilapia (Oreochromis niloticus), Animal Feed Science and Technology, 290, 2022. Crossref