Abonnement à la biblothèque: Guest
Portail numérique Bibliothèque numérique eBooks Revues Références et comptes rendus Collections
International Journal of Medicinal Mushrooms
Facteur d'impact: 1.423 Facteur d'impact sur 5 ans: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Imprimer: 1521-9437
ISSN En ligne: 1940-4344

Volume 21, 2019 Volume 20, 2018 Volume 19, 2017 Volume 18, 2016 Volume 17, 2015 Volume 16, 2014 Volume 15, 2013 Volume 14, 2012 Volume 13, 2011 Volume 12, 2010 Volume 11, 2009 Volume 10, 2008 Volume 9, 2007 Volume 8, 2006 Volume 7, 2005 Volume 6, 2004 Volume 5, 2003 Volume 4, 2002 Volume 3, 2001 Volume 2, 2000 Volume 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushr.v10.i3.40
pages 235-244

Immunomodulation Activity of a Polysaccharide Fraction of a Culinary-Medicinal Mushroom, Pleurotus citrinopileatus Singer (Agaricomycetideae), in Vitro

Ken-ichiro Minato
School of Food, Agricultural, and Environmental Sciences, Miyagi University, Sendai 982-0215, Japan


The hot water soluble extract of Pleurotus citrinopileatus (HWE-P) induced high expression of mRNA of tumor necrosis factor (TNF)-α, which is a proinflammatory cytokine, in the stimulated murine macrophage RAW264. However, unlike lipopolysaccharides (LPS), the extract did not induce the expression of mRNA in other cytokines, such as in IL-12 and IL-10. TNF-α production, which was induced from RAW264 stimulated with HWE-P (1 mg·mL−1), was approximately 400 pg·mL−1. These findings showed that the fruiting body of P. citrinopileatus stimulated an early immunological response of a murine macrophage. Furthermore, we chromatographically separated and purified an active fraction from the crude extract of P. citrinopileatus and measured its immunomodulating activity in cytokine production from the stimulated macrophages. This crude extract was separated into six fractions (HWE-P-I, II, III, IV, V, and VI) by anion exchange chromatography using DEAE-Sepharose gel. Then it was found that the HWE-P-IV fraction, which possessed a molecular mass approximately 450 kDa, showed the strongest immunomodulating activity in TNF-α production (4.9 ng·mL−1) from the macrophages. This fraction was mainly composed of a sugar. Moreover, production of nitric oxide (NO) was detected in the macrophages stimulated with this fraction. The amount of NO produced from stimulated RAW264 was 33.8 μM. These results indicate that a polysaccharide fraction from P. citrinopileatus possesses a potent immunomodulating activity. Thus, this culinary-medicinal species could become an effective component of a functional food.

Articles with similar content:

Macrophages Stimulated by Polysaccharide Purified from Agaricus brasiliensis S.Wasser et al. (Agaricomycetideae) Enhance mRNA Expression of Th1 Cytokine Including IL-12 and 18
International Journal of Medicinal Mushrooms, Vol.5, 2003, issue 4
Nobuhide Fujitake, Sachiko Kawakami, Masashi Mizuno
The Antitumor Activity of Ganoderma lucidum (Curt.:Fr.)P. Karst. (Ling Zhi) (Aphyllophoromycetideae) Polysaccharides Is Related to Tumor Necrosis Factor-α and Interferon-γ
International Journal of Medicinal Mushrooms, Vol.1, 1999, issue 3
Zhi-Bin Lin, Qun-Hao Zhang
Isolation, Purification, and Immunological Activities of a Low-Molecular-Weight Polysaccharide from the Lingzhi or Reishi Medicinal Mushroom Ganoderma lucidum (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.15, 2013, issue 4
Lina Zhu, Yanfang Liu, Yan Yang, Jing-Song Zhang, Shuai Zhou, Xi Luo, Qingjiu Tang
Evaluation of Anticholinesterase and Inflammation Inhibitory Activity of Medicinal Mushroom Phellinus pini (Basidiomycetes) Fruiting Bodies
International Journal of Medicinal Mushrooms, Vol.18, 2016, issue 11
Tae Soo Lee, Jae-hyuk Choi, Jae Kwang Kim, Trung Kien Nguyen, Kyung Hoan Im
Anti-Inflammation Properties of Fruiting Bodies and Submerged Cultured Mycelia of Culinary-Medicinal Higher Basidiomycetes Mushrooms
International Journal of Medicinal Mushrooms, Vol.18, 2016, issue 11
Yuan-Hua Chang, Abed Agbarya, Pei-Hsuan Wu, Solomon P. Wasser, Lan-Min Lin, Jeng-Leun Mau, Yu-Chieh Lin, Mikheil D. Asatiani, Maxim Krakhmalnyi, Rao-Chi Chien, Sophie-Gabrielle Wasser