Abonnement à la biblothèque: Guest
Portail numérique Bibliothèque numérique eBooks Revues Références et comptes rendus Collections
International Journal of Medicinal Mushrooms
Facteur d'impact: 1.423 Facteur d'impact sur 5 ans: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Imprimer: 1521-9437
ISSN En ligne: 1940-4344

Volumes:
Volume 21, 2019 Volume 20, 2018 Volume 19, 2017 Volume 18, 2016 Volume 17, 2015 Volume 16, 2014 Volume 15, 2013 Volume 14, 2012 Volume 13, 2011 Volume 12, 2010 Volume 11, 2009 Volume 10, 2008 Volume 9, 2007 Volume 8, 2006 Volume 7, 2005 Volume 6, 2004 Volume 5, 2003 Volume 4, 2002 Volume 3, 2001 Volume 2, 2000 Volume 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v7.i3.1090
pages 480-481

The Structure of the Polysaccharides Produced by Higher Basidiomyces Tremella mesenterica Ritz.: Fr. and Inonotus levis P. Karst.

Evgeny Vinogradov
Institute for Biological Sciences, National Research Council, Ottawa, Ontario, Canada
Bent O. Petersen
Carlsberg Laboratory, Gamle Carlsberg Vej 10, DK-2500 Valby, Copenhagen, Denmark
Jens O. Duus
Carlsberg Laboratory, Gamle Carlsberg Vej 10, DK-2500 Valby, Copenhagen, Denmark

RÉSUMÉ

Polysaccharides are the best known and most potent mushroom-derived substances, with various positive health effects including immunostimulation, lowering glucose level in blood, anticancer activity, and many others. Biologically active polysaccharides are widerspread among higher Basidiomycetes mushrooms, and most of them have unique structures in different species. There is still no clear correlation between biological activity and structural features of the polysaccharides. This situation results in part from poor knowledge of polysaccharide structures. Herein, we present the results from analyzing two polymers of Tremella mesenterica and Inonotus levis.
Tremella mesenterica (Yellow Brain mushroom) possesses a wide spectrum of medicinal properties, including immunostimulating, protecting against radiation, antidiabetic, anti-inflammatory, hypocholesterolemic, hepatoprotective, and antiallergic effects. Glucuronoxylomannan (GXM), an extracellular polysaccharide produced by single cell culture of T. mesenterica, has a number of beneficial health effects. We developed a new strain of T. mesenterica, CBS 101939, which grows in submerged culture and offers superior yields of one-cell biomass rich in exocellular heteropolysaccharide GXM.
NMR and chemical analysis showed that it has a defined structure of repeating unit, which is O-acetylated at several points, stoichometrically at O-6 of two mannose residues, and partially at GlcA residue, which is present in non-acetylated or acetylated at positions 3 or 4 forms (see Fıg. 1).
These results differ from existing data on the structures of mushroom mannan-based polysaccharides, where mannan backbone was believed to be randomly glycosylated with xylan chains of different lengths.
Inonotus levis polysaccharide had the structure shown in Figure 2, where terminal glucuronic acid residue is present in about half of the molecules, thus making some of the chains acidic and others neutral. We believe that these short polymeric chains were originally attached to some protein via serine or threonine residue and were cleaved off due to alkaline conditions of extraction. Another polymer, co-extracted with this galactan, was a branched, phosphorylated mannan with a structure similar to that of the mannan from yeast Saccharomyces cerevisiae yeast.


Articles with similar content:

The Isolation, Structure, and Applications of the Exocellular Heteropolysaccharide Glucuronoxylomannan Produced by Yellow Brain Mushroom Tremella mesenterica Ritz.:Fr. (Heterobasidiomycetes)
International Journal of Medicinal Mushrooms, Vol.6, 2004, issue 4
Bent O. Petersen, Jens O. Duus, Evgeny Vinogradov
Biomedicinal Triterpenoids of Ganoderma lucidum (Curt.: Fr.) P. Karst. (Aphyllophoromycetideae)
International Journal of Medicinal Mushrooms, Vol.1, 1999, issue 2
Ha Won Kim, Byong Kak Kim
Probing Lingzhi or Reishi Medicinal Mushroom Ganoderma lucidum (Higher Basidiomycetes): A Bitter Mushroom with Amazing Health Benefits
International Journal of Medicinal Mushrooms, Vol.15, 2013, issue 2
Robinka Khajuria, Anil Kumar Sharma, Priya Batra
Comparative Studies on the Influence of Higher Basidiomycetes Polysaccharide Fractions on Reactive Oxidizing Species Production
International Journal of Medicinal Mushrooms, Vol.8, 2006, issue 2
Tajalli Keshavarz, Gopalkrishna Lakkireddy, Christopher Bucke
Ganoderma lucidum (Curt.: Fr.) P. Karst. (Aphyllophoromycetideae) Inhibits Proliferation of Human Peripheral Blood Lymphocytes by Blocking lnterleukin-2 Secretion
International Journal of Medicinal Mushrooms, Vol.2, 2000, issue 4
Ha Won Kim, Byong Kak Kim, Mi Ja Shim