Abonnement à la biblothèque: Guest
Portail numérique Bibliothèque numérique eBooks Revues Références et comptes rendus Collections
International Journal of Medicinal Mushrooms
Facteur d'impact: 1.423 Facteur d'impact sur 5 ans: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Imprimer: 1521-9437
ISSN En ligne: 1940-4344

Volumes:
Volume 21, 2019 Volume 20, 2018 Volume 19, 2017 Volume 18, 2016 Volume 17, 2015 Volume 16, 2014 Volume 15, 2013 Volume 14, 2012 Volume 13, 2011 Volume 12, 2010 Volume 11, 2009 Volume 10, 2008 Volume 9, 2007 Volume 8, 2006 Volume 7, 2005 Volume 6, 2004 Volume 5, 2003 Volume 4, 2002 Volume 3, 2001 Volume 2, 2000 Volume 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v7.i3.560
413 pages

Effect of Substrate Composition and Heat Pretreatment on Vegetative Growth of Agaricus brasiliensis S. Wasser et al. and A. subrufescens Peck

Omoanghe S. Isikhuemhen
Mushroom Biology and Fungal Biotechnology Laboratory, Department of Natural Resources and Environmental Design, College of Agriculture and Environmental Sciences, North Carolina Agricultural and Technical State University, Greensboro, North Carolina
C. Ivey
Mushroom Biology and Fungal Biotechnology Laboratory, School of Agriculture and Environmental Sciences, North Carolina Agricultural and Technical State University, Greensboro, NC 27411, USA

RÉSUMÉ

Approximately 670 million broiler chickens and 43 million turkeys are produced in North Carolina annually, generating wastes that are causing environmental problems and concerns among residents. These waste products can be used as substrate for growing specialty mushrooms. Mushroom farming can enable farmers to obtain additional crops (mushrooms), while bioconverting the agricultural wastes to more environment friendly products such as organic fertilizers. Agaricus brasiliensis and A. subrufescens are culinary−medicinal mushrooms currently produced in the US. These mushrooms can be alternative crops generating high income in the place of tobacco for the North Carolina farmers.
Various combinations of sawdust, poultry litter, and cotton waste were made and loaded in 3 × 12 cm glass test tubes. The tubes containing substrate were sterilized at 121 °C for 1, 2, or 3 hours. A set of 8 tubes per substrate combination was inoculated with A. brasiliensis or A. subrufescens. Similar substrate combinations were pasteurized at 90 °C for 48 hours and then inoculated.
Growth rates of each substrate combination/heat treatment were determined, based on the time for achieving total substrate colonization in each tube. To determine the effect of nutrient supplementation on mycelia growth and development, the substrate combination/heat treatment that performed best in the above treatments was supplemented with wheat bran or millet or combinations of both at 10, 15, and 20% proportions. Non-supplemented substrate served as a control.
Substrate combinations containing cotton waste did not show significant growth and development in either A. brasiliensis or A. subrufescens. Sterilization was ineffective at suppressing contaminants in any substrate combination that had poultry liter. Pasteurization of poultry litter containing substrates at 90 deg;C for 48 hours was sufficient to suppress contaminant. Poultry litter and sawdust in a 1:1 ratio proved to be the best substrate for supporting mycelia growth, where total colonization took an average of 15 days, reaching primordial formation in 60 days. Both fungi responded well to nutrient supplementation, showing an average of 81.5% increase in mycelia growth over the control. In both fungi tested, there was consistent increase in colonization rate as supplementation increased; however, incidence of contaminants increased with the increase of supplementation.
Further work on optimization of substrate pretreatment and environmental conditions that best support the development of primordial through mature fruit body formation in both fungi are currently being researched.


Articles with similar content:

Cultivation of Lentinus squarrosulus (Mont.) Singer on Sawdust of Selected Tropical Tree Species
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 3
Emmanuel Oluwadare Akpaja, Abbot Oghenekaro, John Aroye Okhuoya
Productivity and Nutritional Content of Culinary-Medicinal Oyster Mushroom Pleurotus ostreatus (Jacq.: Fr.) P. Kumm. (Agaricomycetideae) Fruit Bodies Cultivated on Substrates Containing Solid Waste from Anaerobic Digested Poultry Litter
International Journal of Medicinal Mushrooms, Vol.11, 2009, issue 2
Nona A. Mikiashvili, Omoanghe S. Isikhuemhen
The Growing Effect of Vineyard and Winery Wastes on the Production of Mycelia and Fruit Bodies of Edible and Medicinal Fungi
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 3
George Dicu, Alexandru Teodorescu, Marian Petre
Lignocellulolytic Enzyme Activity During Growth and Fruiting of the Edible and Medicinal Mushroom Pleurotus ostreatus (Jacq.:Fr.) Kumm. (Agaricomycetideae)
International Journal of Medicinal Mushrooms, Vol.5, 2003, issue 2
Eva Kachlishvili, Nino Tsiklauri, Tamar Khardziani, David Chichua, Vladimir I. Elisashvili
Effect of Cost-Effective Substrates on Growth Cycle and Yield of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Higher Basidiomycetes) from Northwestern Himalaya (India)
International Journal of Medicinal Mushrooms, Vol.16, 2014, issue 6
Savita Jandaik, Dharmesh Gupta, Sheetal Mehta