Abonnement à la biblothèque: Guest
Portail numérique Bibliothèque numérique eBooks Revues Références et comptes rendus Collections
International Journal of Medicinal Mushrooms
Facteur d'impact: 1.423 Facteur d'impact sur 5 ans: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Imprimer: 1521-9437
ISSN En ligne: 1940-4344

Volumes:
Volume 21, 2019 Volume 20, 2018 Volume 19, 2017 Volume 18, 2016 Volume 17, 2015 Volume 16, 2014 Volume 15, 2013 Volume 14, 2012 Volume 13, 2011 Volume 12, 2010 Volume 11, 2009 Volume 10, 2008 Volume 9, 2007 Volume 8, 2006 Volume 7, 2005 Volume 6, 2004 Volume 5, 2003 Volume 4, 2002 Volume 3, 2001 Volume 2, 2000 Volume 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v7.i3.520
408 pages

Antitumor Immunity in Agaricus sp., Paffia paniculata, and Propolis

Yeunhwa Gu
Department of Radiological Technology, Suzuka University of Medical Science, 1001-1 Kishiokacho, Suzuka City, Mie 510-02, Japan
Takenori Yamashita
Faculty of Health Science, Suzuka University of Medical Science, 1001 Kishioka-cho Suzuka Mie 510-0293, Japan
Masami Oshima
Faculty of Health Science, Suzuka University of Medical Science, 1001 Kishioka-cho Suzuka Mie 510-0293, Japan
Ikukatsu Suzuki
Faculty of Health Science, Suzuka University of Medical Science, 1001 Kishioka-cho Suzuka Mie 510-0293, Japan
Toshihiro Maenaka
Faculty of Health Science, Suzuka University of Medical Science, 1001 Kishioka-cho Suzuka Mie 510-0293, Japan
Norihide Mitsumoto
Shizen Kyusei Co., Ltd, Okayama, Japan

RÉSUMÉ

We investigated the antioxidant activity, lymphocyte versus polymorphonuclear leukocytes enhancement activity (L/P activity), and antitumor activity of three simple substances (Agaricus sp., Paffia paniculata, and Propolis) as well as a mixture of these three kinds of substances (ABP).
A powdered mixture (ABP) of these three substances were prepared using an agate mortar. For the extraction, 2000 mL of water was added to 300 g of finely powdered Agaricus sp. and then stirred for 2 hours in a water bath of 40 °C. After centrifugation for 10 minutes at 5000 rpm, the supernatant was filtered using folded filter paper. Distilled water (2000 mL) was added to the precipitates, and extraction was repeated in the same way. Dried Agaricus sp. was obtained by combining the supernatant from the first extraction with the supernatant from the second extraction (yield: 48.0 g, recovery: 16%). Propolis (100 g) was powdered, and 300 mL of 70% ethanolwas added. After drying at room temperature, filtration was carried out using folded filter paper. The filtrates were dried using an evaporator. Dried Propolis was obtained by freezing and thawing (yield: 53.5 g, recovery: 53.5%). Distilled water (2000 mL) was added to 200 g of finely powdered Paffia paniculata. Dried P. paniculata was obtained by the same technique (yield: 83.6 g, recovery: 41%).
With respect to radical scavenging activity, water and P. paniculata had no radical scavenging activity and showed no marked difference, whereas Agaricus sp. showed slight radical scavenging activity. Both ABP and Propolis showed greater radical scavenging activity than 0.2 mM Trolox, which was used as a positive standard.
Ten neonatal Swiss-Webster mice were divided into two groups. Saline was injected intraperitoneally into one group, and Agaricus sp. was injected intraperitoneally into the other group at a dose of 200 μg/mouse. L/P ratios after the administration of ABP (200 μg/ mouse) were 0.91 ± 0.07 (day 6), 3.23 ± 0.39 (day 10), and 4.82 ± 0.46 (day 14), whereas L/P ratios in the control group were 0.43 ± 0.04 (day 6), 0.96 ± 0.08 (day 10), and 1.43 ± 0.39 (day 14). The L/P ratio after the administration of Propolis was significantly elevated (p < 0.01), as compared to the control group.
When the ABP mixture was administered at a dose of 400 μg/kg for 34 consecutive days, remarkably high antitumor activity (suppressive ratio: 85.1%, p < 0.01) was shown.
In Sarcoma 100 solid carcinoma, when Agaricus sp. (200 mg/kg B.W./day), Paffia paniculata (60 mg/ kg B.W./day), and Propolis (80 mg/kg B.W./day) were orally administered for 34 consecutive days, suppressive ratios were 60.3% (p < 0.05), 54.8% (p < 0.05), and 62.6% (p < 0.05), respectively. When the ABP mixture was administered at a dose of 400 mg/kg B.W./day for 34 consecutive days, remarkably high antitumor activity (suppressive ratio: 83.5%, p < 0.01) was shown.


Articles with similar content:

Toxicological Evaluation of Lentinus squarrosulus Mont. (Polyporales), an Indigenous Nigerian Mushroom
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 3
Mukaila Kadiri
Hypoglycemic Activity of Polysaccharide from Fruiting Bodies of the Shaggy Ink Cap Medicinal Mushroom, Coprinus comatus (Higher Basidiomycetes), on Mice Induced by Alloxan and Its Potential Mechanism
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 10
Yanfang Liu, Yan Yang, Jing-Song Zhang, Shuai Zhou, Qingjiu Tang
Nephroprotective and Antioxidant Effects of King Tuber Oyster Medicinal Mushroom, Pleurotus tuber-regium (Agaricomycetes), on Carbon Tetrachloride-Induced Nephrotoxicity in Male Sprague Dawley Rats
International Journal of Medicinal Mushrooms, Vol.20, 2018, issue 5
Kenneth O. Okolo, Orish Ebere Orisakwe, Iyeopu M. Siminialayi
Aqueous Extract of Wood Ear Mushroom, Auricularia polytricha (Agaricomycetes), Demonstrated Antiepileptic Activity against Seizure Induced by Maximal Electroshock and Isoniazid in Experimental Animals
International Journal of Medicinal Mushrooms, Vol.21, 2019, issue 1
Rajendra Awasthi, Gaurav Gupta, Rakesh Kumar Sharma, Kamal Dua, Rajiv Dahiya, Sachchidanand Pathak, Mohit Agrawal, Anurag Mishra
Protective Effects of Extract from Sclerotium of the King Tuber Medicinal Mushroom, Pleurotus tuberregium (Higher Basidiomycetes) on Carbon Tetrachloride-Induced Hepatotoxicity in Wistar Albino Rats
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 12
Christopher C. Osubor, Chidube A. Alagbaoso, Omoanghe S. Isikhuemhen