Abonnement à la biblothèque: Guest
Portail numérique Bibliothèque numérique eBooks Revues Références et comptes rendus Collections
International Journal of Medicinal Mushrooms
Facteur d'impact: 1.423 Facteur d'impact sur 5 ans: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN Imprimer: 1521-9437
ISSN En ligne: 1940-4344

Volumes:
Volume 21, 2019 Volume 20, 2018 Volume 19, 2017 Volume 18, 2016 Volume 17, 2015 Volume 16, 2014 Volume 15, 2013 Volume 14, 2012 Volume 13, 2011 Volume 12, 2010 Volume 11, 2009 Volume 10, 2008 Volume 9, 2007 Volume 8, 2006 Volume 7, 2005 Volume 6, 2004 Volume 5, 2003 Volume 4, 2002 Volume 3, 2001 Volume 2, 2000 Volume 1, 1999

International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v7.i3.570
414 pages

Spore Germination and Breeding Pattern in Grifola frondosa (Dicks.:Fr.) S.F. Gray

Omoanghe S. Isikhuemhen
Mushroom Biology and Fungal Biotechnology Laboratory, Department of Natural Resources and Environmental Design, College of Agriculture and Environmental Sciences, North Carolina Agricultural and Technical State University, Greensboro, North Carolina
Kasey Vaughans-Ward
Mushroom Biology and Fungal Biotechnology Laboratory, School of Agriculture and Environmental Sciences, North Carolina Agricultural and Technical State University, Greensboro, NC 27411, USA

RÉSUMÉ

Grifola frondosa (Meripiliaceae, Polyporales), commonly called Maitake, grows on hardwood trees in the northern temperate forests of the eastern United States, Canada, Europe, and Asia. It produces sporophores that are edible with high organoleptic properties. In addition to Maitake’s use as food, it is known to exhibit medicinal properties and is thus a fungus of high economic importance today.
The attempt to develop commercial strains from isolates of G. frondosa distributed in the US revealed the difficulties encountered at spore germination. Therefore, it became necessary to investigate various physicochemical factors that can affect spore germination of G. frondosa. Plant hormones, gibberellic acid (GA) and indole-3-acetic acid (IAA), temperature, pH, sonication, mushroom extract, and soil extract were tested in laboratory experiments. Temperature and pH showed significantly higher spore germination values compared to the control (2.8 spores per plate). In 30 °C temperature pretreatment, an average of 28.6 spores germinated at pH 7 resulted in an average germination of 32.2 spores per plate. Some of the concentrations of plant hormones showed germination values that were significantly higher than the control. The concentrations of plant hormones that were not significantly higher than the control were 1.0 mg/L GA, 0.01 mg/L IAA, and 0.05 mg/L IAA. The effect of sonication on spore germination was also significantly higher than the control in all the time periods tested. Soil extract treatment had a negative effect on spore germination as the values were significantly lower than the control. It was therefore concluded that pH and heat pretreatments were the most appropriate factors to stimulate spore germination in .
A preliminary study to determine the breeding pattern in G. frondosa was also conducted. Single spore isolates (ssi) were selected and examined for clamp connections, and 10 clamp free ssi were selected for use in the mating experiment. Cross mating was conducted by co-cultivating pairs of single spore isolates on 2% PDA plates and incubating for 14 days. All ssi were crossed in all possible combinations. Examination to determine formation or absence of clamp connection under ×100 and ×400 magnification conducted on all crosses was conducted, and the results analyzed for the breeding pattern. The results indicated that G. frondosa exhibits a tetra-polar breeding pattern. Among the 10 isolates used, the distribution of mating alles were 2 ssi A1B1, 2 ssi A2B2, 3 ssi A1B2, and 3 ssi A2B1. It was observed that clamp connection formation in G. frondosa is more than 70% and 50% less than what we normally observe in dikaryotic cultures of commercially cultivated species of Pleurotus and Lentinus.


Articles with similar content:

Comparative Study of Worldwide Species of Genus Lentinus (=Lentinula, Higher Basidiomycetes) Based on Linear Mycelium Growth
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 5
Juan Luis Mata, Nutan Tulapurkar Mishra
Genetic Resources and Mycelial Characteristics of Several Medicinal Polypore Mushrooms (Polyporales, Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 4
Alla V. Shnyreva, Susanna M. Badalyan, Alessandra Zambonelli, Mirco Iotti
Preliminary Studies on Mating and Improved Strain Selection in the Tropical Culinary-Medicinal Mushroom Lentinus squarrosulus Mont. (Agaricomycetideae)
International Journal of Medicinal Mushrooms, Vol.12, 2010, issue 2
Elijah I. Ohimain, Omoanghe S. Isikhuemhen, Clementina O. Adenipekun
Cytological Characterization of Anamorphic Fungus Lecanicillium pui and Its Relationship with Chinese Caterpillar Mushroom, Ophiocordyceps sinensis (Ascomycetes)
International Journal of Medicinal Mushrooms, Vol.18, 2016, issue 1
Wei Lei, Guangguo Wu, Guren Zhang, Xin Liu
In Vitro Studies on Interactions Between Strains of Trichoderma spp. and Lentinus edodes (Berk.) Singer Mycelium
International Journal of Medicinal Mushrooms, Vol.7, 2005, issue 3
Olena V. Shulga