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International Journal of Medicinal Mushrooms
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ISSN Imprimer: 1521-9437
ISSN En ligne: 1940-4344

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International Journal of Medicinal Mushrooms

DOI: 10.1615/IntJMedMushrooms.v7.i3.1010
470 pages

Influence of Selected Microelements on the Laccase and Peroxidases Production by Pleurotus eryngii (DC.: Fr.) Quel. in Submerged Cultures

Mirjana Stajic
Institute of Botany and Botanical Garden "Jevremovac", Faculty of Biology, University of Belgrade, Takovska 43, 11000 Belgrade, Serbia
Limor-Leibovitz Persky
Department of Plant Pathology and Microbiology, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University, P. 0. Box 12, Rehovot 76100, Israel
Yitzhak Hadar
Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel
Dana Friesem
Department of Plant Pathology and Microbiology, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University, P.O. Box 12, Rehovot 76100, Israel
Sonja Duletic-Lausevic
Institute of Botany, Faculty of Biology, University of Belgrade, 11000 Belgrade, Serbia
Jelena Vukojević
Institute of Botany and Botanical Garden "Jevremovac", Faculty of Biology, University of Belgrade, Takovska 43, 11000 Belgrade, Serbia

RÉSUMÉ

Pleurotus eryngii var. eryngii is a white-rot fungus because of its ability to produce extracellular ligninolytic enzymes laccase (Lac), two peroxidases (Mn dependent peroxidase, MnP, and versatile peroxidase, VP), and aryl-alcohol oxidase (AAO). Microelements copper (Cu), manganese (Mn), zinc (Zn), iron (Fe), and selenium (Se) influenced the Lac and peroxidase production by this species.
The aim of this investigation was to study the effect of Cu, Mn, Fe, Zn, and Se under conditions of submerged fermentation (SF) of dry ground mandarin peels on the production of Lac and peroxidases in the selected P. eryngii var. eryngii strain.
The culture of P. eryngii var. eryngii, strain No. 616, is preserved in the Culture Collection of the Institute of Evolution, University of Haifa (HAI). The investigated P. eryngii strain was grown under SF conditions of dry ground mandarin peels (as a carbon source) and (NH4)2SO4 (as a nitrogen source). Different combinations and concentrations of Cu, Mn, Fe, Zn, and Se in the forms of: CuSO4 × 7H2O, MnSO4 × H2O, FeSO4 × 7H2O, ZnSO4 × 7H2O, Na2SeO3, Na2SeO4, and SeO2 were analyzed. Ligninolytic enzyme activities were measured after 5 and 7 days of cultivation. Lac and peroxidase activities were determined using syringaldasine and phenol red, respectively. An UV-160A Spectrophotometer (Shimaden) was used for these assays.
The optimum Cu2+ concentration for Lac production was 1 mM (300.12 U/L after 7 days of cultivation), while for peroxidases 10 mM Mn2+ concentration of 5 mM led to peaks of Lac (322.62 U/L, after 7 days of cultivation) and peroxidase activities. However, peroxidase production was significantly lower at Mn2+ concentrations of 1 mM and 3 mM compared to the control. The addition of Zn2+ and Fe2+ to the medium in a concentration of 1 mM led to the significantly high increase of Lac, slight increase of VP, and decrease of MnP activity. Se presented in the concentration of 1 mM, in all investigated forms, caused a decrease of Lac activity, especially when it was added in the form of SeO2, while the lowest decrease was shown in the presence of Na2SeO4. In the presence of all forms of Se, MnP activity decreased after 5 days and increased after 7 days of cultivation in comparison with the control, while VP activity increased, especially when Se was presented in the form of SeO2.


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