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International Journal of Physiology and Pathophysiology
SJR: 0.116

ISSN Imprimer: 2155-014X
ISSN En ligne: 2155-0158

Archives: Volume 1, 2010 to Volume 9, 2018

International Journal of Physiology and Pathophysiology

DOI: 10.1615/IntJPhysPathophys.v8.i1.10
pages 1-12

Net Water Transport via Rat Colon Epithelium under the Experimental Dysbiosis

Taisa Dovbynchuk
Bogomolets National Medical University, Kyiv, Ukraine
Ludmila Zakordonets
Kyiv Taras Shevchenko National University, Kyiv, Ukraine
Andrei Putnikov
Kyiv Taras Shevchenko National University, Kyiv, Ukraine
Ihor Vareniuk
Kyiv Taras Shevchenko National University, Kyiv, Ukraine
Oleksandra Tiapko
Kyiv Taras Shevchenko National University, Kyiv, Ukraine
Nataliya Roslova
Kyiv Taras Shevchenko National University, Kyiv, Ukraine
Tatyana Sergiychuk
Kyiv Taras Shevchenko National University, Kyiv, Ukraine
Oksana V. Lynchak
Kyiv Taras Shevchenko National University, Kyiv, Ukraine
Mycola Dzerzhynsky
Kyiv Taras Shevchenko National University, Kyiv, Ukraine
Tetyana V. Beregova
Kyiv Taras Shevchenko National University, Kyiv, Ukraine; Bogomolets National Medical University, Kyiv, Ukraine
Ganna Tolstanova
Bogomolets National Medical University, Kyiv, Ukraine

RÉSUMÉ

The aim of the study was to investigate the effect of cephalosporin antibiotic ceftriaxone (50 mg/kg, i/m) and macrolide antibiotic azithromycin (15 mg/kg, per.os) on net water transport across rat colonic epithelium. The study was performed on male Wistar rats (180 − 250 g). Azithromycin and ceftriaxone were injected daily for 5 days. Net water transport was evaluated on the 6th day by the isolated colonic loop perfusion technique in vivo on anaesthetized rats. It has been revealed that azithromycin induced a 2.4-fold increase in water absorption, while ceftriaxone caused a 1.9-fold decrease. Five-day-long treatment with antibiotics didn't change the composition of the fecal and colonic parietal microbiota. Azithromycin-induced increase in water absorption was associated with upregulation of Aquaporin-8 (AQP 8) water channel expression (P < 0.05) in the rat colonic mucosa. Conversely, ceftriaxone-induced decrease in water absorption did not affect the protein expression of AQP-8, but it resulted in pro-inflammatory changes in the structure of colonic mucosa and mast cells degranulation. Thus, we have shown for the first time the opposit effects of macrolide and cephalosporin antibiotics on the net water transport across the rat colonic epithelium.